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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HMGCL Recombinant antibody

HMGCL Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242224G10

Cat no : 84769-1-RR

Synonyms

HMG-CoA lyase, HMG CoA lyase, HL, EC:4.1.3.4, 242224G10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HMGCL Recombinant antibody (84769-1-RR)

    WB analysis using 84769-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84769-1-RR (HMGCL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84769-1-RR

    Affinity and Kinetic Characterization of 84769-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84769-1-RR against Human HMGCL were performed. The affinity constant is 0.408 nM.

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Tested Applications

Positive WB detected inHepG2 cells, K-562 cells, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84769-1-RR targets HMGCL in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HMGCL fusion protein Ag10426 相同性解析による交差性が予測される生物種
Full Name 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase
Calculated molecular weight 325 aa, 34 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC010570
Gene symbol HMGCL
Gene ID (NCBI) 3155
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HMGCL (3-hydroxy-3-methylglutaryl-CoA lyase) is a key enzyme in ketogenesis (ketone body formation). It is a ketogenic enzyme in the liver that catalyzes the formation of acetoacetate from HMG-CoA within the mitochondria. It also plays a prominent role in the catabolism of the amino acid leucine.

Protocols

Product Specific Protocols
WB protocol for HMGCL antibody 84769-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols