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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using HMGN4 Polyclonal antibody (11686-1-AP)

    WB analysis of HeLa using 11686-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11686-1-AP (HMGN4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HMGN4 Polyclonal antibody (11686-1-AP)

    IF Staining of HeLa using 11686-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HMGN4 antibody (11686-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

HMGN4 Polyclonal antibody

HMGN4 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 11686-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Non-histone chromosomal protein HMG-17-like 3, Non-histone chromosomal protein, NHC, HMG17L3, High mobility group nucleosome-binding domain-containing protein 4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11686-1-AP targets HMGN4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HMGN4 fusion protein Ag2298 相同性解析による交差性が予測される生物種
Full Name high mobility group nucleosomal binding domain 4
Calculated molecular weight 90 aa, 10 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC001282
Gene Symbol HMGN4
Gene ID (NCBI) 10473
RRIDAB_2118489
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00479
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HMGN4, also named as High mobility group nucleosome-binding domain-containing protein 4, is a 90 amino acid protein, which belongs to the HMGN family. HMGN4 as a DNA-binding protein localizes in the nucleus. The calculated molecular weight of HMGN4 is 10 kDa, but the modified HMGN4 protein is about 15 kDa.

Protocols

Product Specific Protocols
WB protocol for HMGN4 antibody 11686-1-APDownload protocol
IF protocol for HMGN4 antibody 11686-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols