Validation Data Gallery

Filter: AllWBIPIHCIF-PFC (Intra)

WB analysis of HeLa using 10701-1-AP
WB result of HMOX1 antibody (10701-1-AP, 1:3000) with si-Control and si-HMOX1 transfected HeLa cells.
WB analysis using 10701-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10701-1-AP (HO-1/HMOX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 10701-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10701-1-AP (HO-1/HMOX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 10701-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10701-1-AP (HO-1/HMOX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 10701-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10701-1-AP (HMOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10701-1-AP
IP result of anti-HO-1/HMOX1 (IP:10701-1-AP, 4ug; Detection:10701-1-AP 1:20000) with HeLa cells lysate 1360 ug.
IHC staining of mouse spleen using 10701-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 10701-1-AP (HO-1/HMOX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse spleen using 10701-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 10701-1-AP (HO-1/HMOX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse spleen using 10701-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using HO-1/HMOX1 antibody (10701-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse spleen using 10701-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using HO-1/HMOX1 antibody (10701-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse spleen using 10701-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using HO-1/HMOX1 antibody (10701-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC experiment of HeLa using 10701-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human HO-1/HMOX1 (10701-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

HO-1/HMOX1 Polyclonal antibody

HO-1/HMOX1 Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), IP, ELISA

Cat No. 10701-1-AP

Publications(1127)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IHC, IF-P, FC (Intra), IP, CoIP, ELISA

Synonyms

HMOX1, HO-1, Heme oxygenase 1 soluble form, Heme oxygenase 1, heme oxygenase (decycling) 1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (1127)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HT-1080 cells, HeLa cells, rat spleen tissue, HepG2 cells, mouse liver tissue, rat liver tissue, mouse kidney tissue, mouse spleen tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse spleen tissue
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 22 publications below
WB	See 1069 publications below
IHC	See 89 publications below
IF	See 84 publications below
IP	See 2 publications below
FC	See 2 publications below
CoIP	See 2 publications below

Product Information

10701-1-AP targets HO-1/HMOX1 in WB, IHC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, monkey, chicken, bovine, goat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HO-1/HMOX1 fusion protein Ag1190 相同性解析による交差性が予測される生物種
Full Name	heme oxygenase (decycling) 1
Calculated molecular weight	33 kDa
Observed molecular weight	28-33 kDa
GenBank accession number	BC001491
Gene Symbol	HO-1
Gene ID (NCBI)	3162
RRID	AB_2118685
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P09601
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663). And the full-length HO-1 is very unstable and susceptible to truncation that generates an inactive, soluble form (28 kDa) (James R. Reed, Pharmacology, 535-568).

Protocols

Product Specific Protocols
WB protocol for HO-1/HMOX1 antibody 10701-1-AP	Download protocol
IHC protocol for HO-1/HMOX1 antibody 10701-1-AP	Download protocol
IF protocol for HO-1/HMOX1 antibody 10701-1-AP	Download protocol
IP protocol for HO-1/HMOX1 antibody 10701-1-AP	Download protocol
FC protocol for HO-1/HMOX1 antibody 10701-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Res MFSD7c functions as a transporter of choline at the blood-brain barrier Authors - Xuan Thi Anh Nguyen View Article
human	WB	Nature SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells. Authors - Ying Wang View Article
	WB	Cell Metab Integrative genetic analysis identifies FLVCR1 as a plasma-membrane choline transporter in mammals Authors - Timothy C Kenny View Article
	WB	Gut Histone methyltransferase Suv39h1 regulates hepatic stellate cell activation and is targetable in liver fibrosis Authors - Ming Kong View Article
mouse	WB	Cell Metab Functional Genomics In Vivo Reveal Metabolic Dependencies of Pancreatic Cancer Cells. Authors - Xiphias Ge Zhu View Article
mouse,rat	WB,IHC	Bioact Mater A two-pronged approach to inhibit ferroptosis of MSCs caused by the iron overload in postmenopausal osteoporosis and promote osseointegration of titanium implant Authors - Yulu Yang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 10701-1-AP

WB analysis using 10701-1-AP

WB analysis using 10701-1-AP

WB analysis using 10701-1-AP

WB analysis using 10701-1-AP

IP experiment of HeLa using 10701-1-AP

IHC staining of mouse spleen using 10701-1-AP

IHC staining of mouse spleen using 10701-1-AP

IF Staining of mouse spleen using 10701-1-AP

IF Staining of mouse spleen using 10701-1-AP

IF Staining of mouse spleen using 10701-1-AP

FC experiment of HeLa using 10701-1-AP

HO-1/HMOX1 Polyclonal antibody

HO-1/HMOX1 Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

MFSD7c functions as a transporter of choline at the blood-brain barrier

SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells.

Integrative genetic analysis identifies FLVCR1 as a plasma-membrane choline transporter in mammals

Histone methyltransferase Suv39h1 regulates hepatic stellate cell activation and is targetable in liver fibrosis

Functional Genomics In Vivo Reveal Metabolic Dependencies of Pancreatic Cancer Cells.

A two-pronged approach to inhibit ferroptosis of MSCs caused by the iron overload in postmenopausal osteoporosis and promote osseointegration of titanium implant