HMOX2 Polyclonal antibody

Name: HMOX2 Polyclonal antibody
Brand: Proteintech
SKU: 14817-1-AP

HMOX2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14817-1-AP

Synonyms

HO2, HO 2, Heme oxygenase 2 soluble form, Heme oxygenase 2, heme oxygenase (decycling) 2

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of HepG2 using 14817-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 14817-1-AP (HMOX2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 14817-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14817-1-AP (HMOX2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.
WB analysis of HepG2 using 14817-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 14817-1-AP (HMOX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 14817-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 14817-1-AP (HMOX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 14817-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 14817-1-AP (HMOX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 14817-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 14817-1-AP (HMOX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of Raji using 14817-1-AP
IP result of anti-HMOX2 (IP:14817-1-AP, 4ug; Detection:14817-1-AP 1:500) with Raji cells lysate 2000ug.
IHC staining of human lymphoma using 14817-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14817-1-AP (HMOX2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 14817-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14817-1-AP (HMOX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 14817-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HMOX2 antibody (14817-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of HeLa using 14817-1-AP
Immunofluorescent analysis of Hela cells, using HMOX2 antibody 14817-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Cat No. 14817-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HepG2 cells, K-562 cells, mouse spleen tissue, Raji cells
Positive IP detected in	Raji cells
Positive IHC detected in	human lymphoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 6 publications below
IHC	See 1 publications below

Product Information

14817-1-AP targets HMOX2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HMOX2 fusion protein Ag6575 相同性解析による交差性が予測される生物種
Full Name	heme oxygenase (decycling) 2
Calculated molecular weight	36 kDa
Observed molecular weight	36 kDa
GenBank accession number	BC002396
Gene symbol	HMOX2
Gene ID (NCBI)	3163
RRID	AB_2118843
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Heme oxygenase is the rate-limiting enzyme in the heme degradative pathway. There are 2 isozymic forms of heme oxygenase, an inducible heme oxygenase-1 (HMOX1) and a constitutive heme oxygenase-2 (HMOX2). Down-regulation of HMOX2 is associated with the increased expression of HMOX1 in human cell lines. HMOX2 is also named as HO-2 and could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter.

Protocols

Product Specific Protocols
WB protocol for HMOX2 antibody 14817-1-AP	Download protocol
IHC protocol for HMOX2 antibody 14817-1-AP	Download protocol
IF protocol for HMOX2 antibody 14817-1-AP	Download protocol
IP protocol for HMOX2 antibody 14817-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cell Metabolic-scale gene activation screens identify SLCO2B1 as a heme transporter that enhances cellular iron availability. Authors - Gokhan Unlu View Article
rat	WB	Acta Pharm Sin B Organic carbon monoxide prodrug, BW-CO-111, in protection against chemically-induced gastric mucosal damage. Authors - Dominik Bakalarz View Article
human	WB	J Cell Sci Role of epithelial sodium channels (ENaCs) in endothelial function. Authors - Dongqing Guo View Article
human,mouse	WB,IHC	Oxid Med Cell Longev Hydrogen Sulfide Abrogates Hemoglobin-Lipid Interaction in Atherosclerotic Lesion. Authors - László Potor View Article
rat	WB	Cells Evidence for Cytoprotective Effect of Carbon Monoxide Donor in the Development of Acute Esophagitis Leading to Acute Esophageal Epithelium Lesions. Authors - Katarzyna Magierowska View Article
rat	WB	PLoS One Carbon Monoxide (CO) Released from Tricarbonyldichlororuthenium (II) Dimer (CORM-2) in Gastroprotection against Experimental Ethanol-Induced Gastric Damage. Authors - Katarzyna Magierowska View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HMOX2 Polyclonal antibody

HMOX2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HepG2 using 14817-1-AP

WB analysis of mouse spleen using 14817-1-AP

WB analysis of HepG2 using 14817-1-AP

WB analysis of K-562 using 14817-1-AP

WB analysis of Raji using 14817-1-AP

WB analysis of K-562 using 14817-1-AP

IP experiment of Raji using 14817-1-AP

IHC staining of human lymphoma using 14817-1-AP

IHC staining of human lymphoma using 14817-1-AP

IF Staining of HeLa using 14817-1-AP

IF Staining of HeLa using 14817-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Metabolic-scale gene activation screens identify SLCO2B1 as a heme transporter that enhances cellular iron availability.

Organic carbon monoxide prodrug, BW-CO-111, in protection against chemically-induced gastric mucosal damage.

Role of epithelial sodium channels (ENaCs) in endothelial function.

Hydrogen Sulfide Abrogates Hemoglobin-Lipid Interaction in Atherosclerotic Lesion.

Evidence for Cytoprotective Effect of Carbon Monoxide Donor in the Development of Acute Esophagitis Leading to Acute Esophageal Epithelium Lesions.

Carbon Monoxide (CO) Released from Tricarbonyldichlororuthenium (II) Dimer (CORM-2) in Gastroprotection against Experimental Ethanol-Induced Gastric Damage.