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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HMP19 Polyclonal antibody

HMP19 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 19423-1-AP

Synonyms

HMP19, HMP19 protein, NSG2, Protein p19

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using HMP19 Polyclonal antibody (19423-1-AP)

    IHC staining of human hepatocirrhosis using 19423-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19423-1-AP (HMP19 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using HMP19 Polyclonal antibody (19423-1-AP)

    IHC staining of human hepatocirrhosis using 19423-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19423-1-AP (HMP19 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HMP19 Polyclonal antibody (19423-1-AP)

    IF Staining of HepG2 using 19423-1-AP

    Immunofluorescent analysis of HepG2 cells, using HMP19 antibody 19423-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive IHC detected inhuman hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 19423-1-AP is HMP19 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HMP19 fusion protein Ag13751 相同性解析による交差性が予測される生物種
Full Name HMP19 protein
Calculated molecular weight 171 aa, 19 kDa
GenBank accession numberBC002619
Gene symbol HMP19
Gene ID (NCBI) 51617
RRIDAB_10638444
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for HMP19 antibody 19423-1-APDownload protocol
IF protocol for HMP19 antibody 19423-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Int J Cancer

Suppression of pancreatic cancer growth and metastasis by HMP19 identified through genome-wide shRNA screen.

Authors - Hiroshi Kurahara
View Article