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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HN1L Polyclonal antibody

HN1L Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16285-1-AP

Synonyms

Jupiter microtubule associated homolog 2, JPT2, HN1-like protein, Hematological and neurological expressed 1-like protein, C16orf34

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HN1L Polyclonal antibody (16285-1-AP)

    WB analysis using 16285-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16285-1-AP (HN1L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using HN1L Polyclonal antibody (16285-1-AP)

    IF Staining of A431 using 16285-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using HN1L antibody (16285-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16285-1-AP targets HN1L in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HN1L fusion protein Ag9400 相同性解析による交差性が予測される生物種
Full Name hematological and neurological expressed 1-like
Calculated molecular weight 190 aa, 20 kDa
Observed molecular weight20 kDa
GenBank accession numberBC014438
Gene symbol HN1L
Gene ID (NCBI) 90861
RRIDAB_3085484
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Hematological and neurological expressed 1-like protein (HN1L), also known as Jupiter microtubule-associated homolog 2 (JPT2), belongs to the jupiter family. HN1L is nicotinic acid adenine dinucleotide phosphate (NAADP) binding protein required for NAADP-evoked intracellular calcium release (PubMed:33758061). HN1L enables NAADP to activate Ca2+ release from the endoplasmic reticulum through ryanodine receptors (PubMed:33758062). HN1L was also required for the translocation of a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pseudovirus through the endolysosomal systemHN1L is expressed in liver, kidney, prostate, testis and uterus (PMID: 15094197).

Protocols

Product Specific Protocols
WB protocol for HN1L antibody 16285-1-APDownload protocol
IF protocol for HN1L antibody 16285-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols