• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HNF4A Recombinant antibody, PBS Only (Detector)

HNF4A Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241593C4

Cat no : 84316-3-PBS

Synonyms

HNF4a8, HNF4a7, HNF4, HNF 4 alpha, Hepatocyte nuclear factor 4-alpha

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HepG2 cells using HNF4A Recombinant antibody (84316-3-RR)

    WB analysis of HepG2 using 84316-3-RR (same clone as 84316-3-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 84316-3-RR (HNF4A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84316-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01183-3

    Cytometric bead array standard curve of MP01183-3

    Cytometric bead array standard curve of MP01183-3, HNF4A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84316-2-PBS. Detection antibody: 84316-3-PBS. Standard: Ag24647. Range: 0.781-100 ng/mL.

☆ 2/28（金）まで！最大30%オフ！ >> トライアルサイズもお得な一次抗体キャンペーン

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84316-3-PBS targets HNF4A as part of a matched antibody pair:

MP01183-3: 84316-2-PBS capture and 84316-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HNF4A fusion protein Ag24647 相同性解析による交差性が予測される生物種
Full Name hepatocyte nuclear factor 4, alpha
Calculated molecular weight 53 kDa
Observed molecular weight56 kDa
GenBank accession numberBC137539
Gene symbol HNF4A
Gene ID (NCBI) 3172
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HNF4A (Hepatocyte nuclear factor 4 α) is highly conserved between humans and mice. Using conditional knockouts, epithelial HNF4A is indispensable for protecting against acute chemical-induced colitis and spontaneous colonic inflammation in aged animals (PMID:35792863). Moreover, reduced expression of HNF4A has been reported in liver fibrosis and viral vector-mediated HNF4A restoration has previously been demonstrated to reverse features of chronic liver injury. Therefore, HNF4A has been suggested as a therapeutic target for treating liver fibrosis (PMID:34453962).