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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HNF4A Recombinant antibody

HNF4A Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241593A2

Cat no : 84316-7-RR

Synonyms

HNF4a8, HNF4a7, HNF4, HNF 4 alpha, Hepatocyte nuclear factor 4-alpha

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HNF4A Recombinant antibody (84316-7-RR)

    IF Staining of HepG2 using 84316-7-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNF4A antibody (84316-7-RR, Clone: 241593A2 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HNF4A Recombinant antibody (84316-7-RR)

    IF Staining of HeLa using 84316-7-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNF4A antibody (84316-7-RR, Clone: 241593A2 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84316-7-RR

    Affinity and Kinetic Characterization of 84316-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84316-7-RR against Human HNF4A were performed. The affinity constant is 0.275 nM.

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Tested Applications

Positive IF/ICC detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84316-7-RR is HNF4A Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HNF4A fusion protein Ag24647 相同性解析による交差性が予測される生物種
Full Name hepatocyte nuclear factor 4, alpha
Calculated molecular weight 53 kDa
GenBank accession numberBC137539
Gene symbol HNF4A
Gene ID (NCBI) 3172
RRIDAB_3671857
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HNF4A (Hepatocyte nuclear factor 4 α) is highly conserved between humans and mice. Using conditional knockouts, epithelial HNF4A is indispensable for protecting against acute chemical-induced colitis and spontaneous colonic inflammation in aged animals (PMID:35792863). Moreover, reduced expression of HNF4A has been reported in liver fibrosis and viral vector-mediated HNF4A restoration has previously been demonstrated to reverse features of chronic liver injury. Therefore, HNF4A has been suggested as a therapeutic target for the treatment of liver fibrosis (PMID:34453962).

Protocols

Product Specific Protocols
IF protocol for HNF4A antibody 84316-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols