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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HNRNPA0 Polyclonal antibody

HNRNPA0 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10848-1-AP

Synonyms

HNRPA0, hnRNP A0

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using HNRNPA0 Polyclonal antibody (10848-1-AP)

    WB analysis using 10848-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using HNRNPA0 Polyclonal antibody (10848-1-AP)

    WB analysis of HeLa using 10848-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using HNRNPA0 Polyclonal antibody (10848-1-AP)

    WB analysis of mouse brain using 10848-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using HNRNPA0 Polyclonal antibody (10848-1-AP)

    WB analysis of HeLa using 10848-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using HNRNPA0 Polyclonal antibody (10848-1-AP)

    WB analysis of HeLa using 10848-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using HNRNPA0 Polyclonal antibody (10848-1-AP)

    WB analysis of human brain using 10848-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using HNRNPA0 Polyclonal antibody (10848-1-AP)

    WB analysis of fetal human brain using 10848-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of HeLa cells using HNRNPA0 Polyclonal antibody (10848-1-AP)

    WB analysis of HeLa using 10848-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of HeLa cells using HNRNPA0 Polyclonal antibody (10848-1-AP)

    WB analysis of HeLa using 10848-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of human brain tissue using HNRNPA0 Polyclonal antibody (10848-1-AP)

    WB analysis of human brain using 10848-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using HNRNPA0 Polyclonal antibody (10848-1-AP)

    IP experiment of mouse brain using 10848-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 antibody) at dilution of 1:500.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HNRNPA0 Polyclonal antibody (10848-1-AP)

    IF Staining of HeLa using 10848-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 10848-1-AP (HNRNPA0 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, fetal human brain tissue, mouse brain tissue, Jurkat cells, NIH/3T3 cells
Positive IP detected inmouse brain tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10848-1-AP targets HNRNPA0 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HNRNPA0 fusion protein Ag1312 相同性解析による交差性が予測される生物種
Full Name heterogeneous nuclear ribonucleoprotein A0
Calculated molecular weight 31 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC019271
Gene symbol HNRNPA0
Gene ID (NCBI) 10949
RRIDAB_2117066
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HNRNPA0 belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Protocols

Product Specific Protocols
WB protocol for HNRNPA0 antibody 10848-1-APDownload protocol
IF protocol for HNRNPA0 antibody 10848-1-APDownload protocol
IP protocol for HNRNPA0 antibody 10848-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Nat Struct Mol Biol

TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.

Authors - Xinxin Zuo
View Article
humanIF

Cell

RNA damage compartmentalization by DHX9 stress granules

Authors - Yilong Zhou
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Xiaochen (Verified Customer) (07-31-2023)

hnrnpa0 has many subtypes and thus got two bands, I think it is acceptable. Antibody quality is prety good.

  • Applications: Western Blot
HNRNPA0 Antibody Western Blot validation ( dilution) in (Cat no:10848-1-AP)