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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HNRNPA1 Monoclonal antibody

HNRNPA1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A5A4

Cat no : 67844-1-Ig

Synonyms

hnRNP core protein A1, hnRNP A1, Heterogeneous nuclear ribonucleoprotein A1, N-terminally processed, Helix-destabilizing protein, Helix destabilizing protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HNRNPA1 Monoclonal antibody (67844-1-Ig)

    WB analysis using 67844-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of NIH/3T3 cells using HNRNPA1 Monoclonal antibody (67844-1-Ig)

    WB analysis of NIH/3T3 using 67844-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using HNRNPA1 Monoclonal antibody (67844-1-Ig)

    WB analysis of RAW 264.7 using 67844-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HNRNPA1 Monoclonal antibody (67844-1-Ig)

    IHC staining of human lung cancer using 67844-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HNRNPA1 Monoclonal antibody (67844-1-Ig)

    IHC staining of human lung cancer using 67844-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HNRNPA1 Monoclonal antibody (67844-1-Ig)

    IHC staining of human breast cancer using 67844-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HNRNPA1 Monoclonal antibody (67844-1-Ig)

    IHC staining of human liver cancer using 67844-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HNRNPA1 Monoclonal antibody (67844-1-Ig)

    IHC staining of human liver cancer using 67844-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HNRNPA1 Monoclonal antibody (67844-1-Ig)

    IHC staining of human colon cancer using 67844-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HNRNPA1 Monoclonal antibody (67844-1-Ig)

    IHC staining of human colon cancer using 67844-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HNRNPA1 Monoclonal antibody (67844-1-Ig)

    IHC staining of human colon cancer using 67844-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HNRNPA1 Monoclonal antibody (67844-1-Ig)

    IHC staining of human colon cancer using 67844-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HNRNPA1 Monoclonal antibody (67844-1-Ig)

    IF Staining of HeLa using 67844-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPA1 antibody (67844-1-Ig, Clone: 1A5A4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inLNCaP cells, NIH/3T3 cells, RAW 264.7 cells, HeLa cells, Jurkat cells, K-562 cells, HEK-293 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue, human colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67844-1-Ig targets HNRNPA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HNRNPA1 fusion protein Ag17415 相同性解析による交差性が予測される生物種
Full Name heterogeneous nuclear ribonucleoprotein A1
Calculated molecular weight 34 kDa
Observed molecular weight34 kDa
GenBank accession numberBC009600
Gene symbol HNRNPA1
Gene ID (NCBI) 3178
RRIDAB_2918606
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

In eukaryotic cells, nascent RNA polymerase II transcripts are associated in the nucleus with specific proteins to form ribonucleoprotein complexes called HNRP or 40S. Protein moiety of the HNRP particle has 6 major components called core proteins, and HNRPA1 is one of them. hnRNP A1 is a multifunctional RNA binding protein which is involved in pre-mRNA splicing, export of mature transcripts from the nucleus, mRNA turnover, and internal ribosome entry site (IRES)-mediated translation. HNRNPA1 has three isoforms with MW 30 kDa, 34 kDa and 39 kDa.

Protocols

Product Specific Protocols
WB protocol for HNRNPA1 antibody 67844-1-IgDownload protocol
IHC protocol for HNRNPA1 antibody 67844-1-IgDownload protocol
IF protocol for HNRNPA1 antibody 67844-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols