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  • Featured Product
  • KD/KO Validated

HNRNPA2B1 Monoclonal antibody, PBS Only

HNRNPA2B1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3H6F7

Cat no : 67445-1-PBS

Synonyms

DKFZp779B0244, FLJ22720, hnRNP A2/B1, HNRNPA2, HNRNPA2B1, HNRNPB1, HNRPA2, HNRPA2B1, HNRPB1, RNPA2, SNRPB1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HNRNPA2B1 Monoclonal antibody (67445-1-Ig)

    WB analysis using 67445-1-Ig (same clone as 67445-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67445-1-Ig (HNRNPA2B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67445-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using HNRNPA2B1 Monoclonal antibody (67445-1-Ig)

    WB analysis of A549 using 67445-1-Ig (same clone as 67445-1-PBS)

    WB result of HNRNPA2B1 antibody (67445-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HNRNPA2B1 transfected A549 cells. This data was developed using the same antibody clone with 67445-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using HNRNPA2B1 Monoclonal antibody (67445-1-Ig)

    WB analysis using 67445-1-Ig (same clone as 67445-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67445-1-Ig (HNRNPA2B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67445-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using HNRNPA2B1 Monoclonal antibody (67445-1-Ig)

    IHC staining of human colon using 67445-1-Ig (same clone as 67445-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67445-1-Ig (HNRNPA2B1 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67445-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HNRNPA2B1 Monoclonal antibody (67445-1-Ig)

    IHC staining of human lung cancer using 67445-1-Ig (same clone as 67445-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67445-1-Ig (HNRNPA2B1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67445-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HNRNPA2B1 Monoclonal antibody (67445-1-Ig)

    IHC staining of human lung cancer using 67445-1-Ig (same clone as 67445-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67445-1-Ig (HNRNPA2B1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67445-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using HNRNPA2B1 Monoclonal antibody (67445-1-Ig)

    IHC staining of human colon using 67445-1-Ig (same clone as 67445-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67445-1-Ig (HNRNPA2B1 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67445-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using HNRNPA2B1 Monoclonal antibody (67445-1-Ig)

    IF Staining of U2OS using 67445-1-Ig (same clone as 67445-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using HNRNPA2B1 antibody (67445-1-Ig, Clone: 3H6F7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67445-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using HNRNPA2B1 Monoclonal antibody (67445-1-Ig)

    IF Staining of U2OS using 67445-1-Ig (same clone as 67445-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using HNRNPA2B1 antibody (67445-1-Ig, Clone: 3H6F7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67445-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67445-1-PBS targets HNRNPA2B1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen HNRNPA2B1 fusion protein Ag6699 相同性解析による交差性が予測される生物種
Full Name heterogeneous nuclear ribonucleoprotein A2/B1
Calculated molecular weight 37 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC000506
Gene symbol HNRNPA2B1
Gene ID (NCBI) 3181
RRIDAB_2882679
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HnRNPA2B1 is one of heterogenous nuclear ribonucleoproteins (HnRNPs), which regulate the splicing and transportation of mRNA and participate in carcinogenesis. It has a role in packaging nascent mRNA, alternative splicing, tycoplamic RNA trafficking, translation and stabilization. Besides it may involve in telomere maintenance, cell proliferation and differentiation, and glucose transport.