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  • Featured Product
  • KD/KO Validated

HNRNPA3 Polyclonal antibody

HNRNPA3 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugate

Unconjugated

Cat no : 25142-1-AP

Synonyms

HNRPA3, HNRNPA 3, hnRNP A3, FBRNP

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using HNRNPA3 Polyclonal antibody (25142-1-AP)

    WB analysis of Jurkat using 25142-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25142-1-AP (HNRNPA3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using HNRNPA3 Polyclonal antibody (25142-1-AP)

    WB analysis of mouse liver using 25142-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 25142-1-AP (HNRNPA3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using HNRNPA3 Polyclonal antibody (25142-1-AP)

    WB analysis of mouse lung using 25142-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 25142-1-AP (HNRNPA3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using HNRNPA3 Polyclonal antibody (25142-1-AP)

    WB analysis of HeLa using 25142-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25142-1-AP (HNRNPA3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using HNRNPA3 Polyclonal antibody (25142-1-AP)

    IHC staining of human stomach using 25142-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25142-1-AP (HNRNPA3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon using HNRNPA3 Polyclonal antibody (25142-1-AP)

    IHC staining of human colon using 25142-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon slide using 25142-1-AP (HNRNPA3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using HNRNPA3 Polyclonal antibody (25142-1-AP)

    IF Staining of mouse colon using 25142-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using HNRNPA3 antibody (25142-1-AP) at dilution of 1:200, E-cadherin antibody (20874-1-AP, Magenta), CoraLite®594 smooth muscle actin antibody (CL594-14395, red), CD45 antibody (80297-1-RR, Clone: 6O19, orange).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HNRNPA3 Polyclonal antibody (25142-1-AP)

    IF Staining of HeLa using 25142-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPA3 antibody (25142-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inmouse liver tissue, Jurkat cells
Positive IHC detected inhuman stomach tissue, human colon
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse colon tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25142-1-AP targets HNRNPA3 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, pig, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HNRNPA3 fusion protein Ag18248 相同性解析による交差性が予測される生物種
Full Name heterogeneous nuclear ribonucleoprotein A3
Calculated molecular weight 378 aa, 40 kDa
Observed molecular weight 37-40 kDa
GenBank accession numberBC113470
Gene symbol HNRNPA3
Gene ID (NCBI) 220988
RRIDAB_2879921
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HNRNPA3 also named as hnRNP A3 or heterogeneous nuclear ribonucleoprotein A3 is a 378 amino acid protein, which contains 2 RRM domains and localizes in nucleus. HNRNPA3 is a component of the spliceosome C complex and play a role in cytoplasmic trafficking of RNA. Binds to the cis-acting response element.

Protocols

Product Specific Protocols
WB protocol for HNRNPA3 antibody 25142-1-APDownload protocol
IHC protocol for HNRNPA3 antibody 25142-1-APDownload protocol
IF protocol for HNRNPA3 antibody 25142-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

A network of RNA-binding proteins controls translation efficiency to activate anaerobic metabolism.

Authors - J J David Ho
View Article
humanWB

J Cell Biol

Stress granules regulate stress-induced paraspeckle assembly.

Authors - Haiyan An
View Article

Arterioscler Thromb Vasc Biol

Novel Long Noncoding RNA, Macrophage Inflammation-Suppressing Transcript (MIST), Regulates Macrophage Activation During Obesity.

Authors - Kenneth Stapleton
View Article
mouseIP,IF,IHC

Neurobiol Dis

ALS-linked cytoplasmic FUS assemblies are compositionally different from physiological stress granules and sequester hnRNPA3, a novel modifier of FUS toxicity.

Authors - Haiyan An
View Article
humanWB,IF

Sci China Life Sci

Identification of mecciRNAs and their roles in the mitochondrial entry of proteins.

Authors - Xu Liu
View Article
mouseWB

Mediators Inflamm

Integrated Functional Analysis of the Nuclear Proteome of Classically and Alternatively Activated Macrophages.

Authors - John E Wiktorowicz
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Tatyana (Verified Customer) (01-21-2023)

Works well in ICC and WB in human cells. Also gives a weak but specific signal in the mouse CNS tissue (spinal cord). WB: 4% milk in TBST and semi-dry transfer; detects a double band at ~55kDa ICC: 5% goat serum in PBST; mainly nuclear IHC: 4% PFA fixed and paraffinised sections; standard antigen retrieval and DAB detection; mainly nuclear Used at 1:1000, with incubation overnight at 4C, in all applications

  • Applications: Western Blot, Immunohistochemistry, Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human / SH-SY5Y cells; mouse / spinal cord
HNRNPA3 Antibody Western Blot,Immunohistochemistry,Immunofluorescence validation (1:1000 dilution) in Human / SH-SY5Y cells; mouse / spinal cord (Cat no:25142-1-AP)