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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HNRNPAB Recombinant antibody (84207-1-RR)

    WB analysis using 84207-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84207-1-RR (HNRNPAB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using HNRNPAB Recombinant antibody (84207-1-RR)

    WB analysis of HeLa using 84207-1-RR

    WB result of HNRNPAB antibody (84207-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HNRNPAB transfected HeLa cells.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HNRNPAB Recombinant antibody (84207-1-RR)

    IF Staining of HeLa using 84207-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPAB antibody (84207-1-RR, Clone: 241462B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HNRNPAB Recombinant antibody (84207-1-RR)

    IF Staining of HeLa using 84207-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPAB antibody (84207-1-RR, Clone: 241462B1 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

  • Flow cytometry (FC) experiment of Jurkat cells using HNRNPAB Recombinant antibody (84207-1-RR)

    FC experiment of Jurkat using 84207-1-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug HNRNPAB Recombinant antibody (84207-1-RR, Clone:241462B1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Flow cytometry (FC) experiment of HeLa cells using HNRNPAB Recombinant antibody (84207-1-RR)

    FC experiment of HeLa using 84207-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HNRNPAB Recombinant antibody (84207-1-RR, Clone:241462B1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 84207-1-RR

    Affinity and Kinetic Characterization of 84207-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84207-1-RR against Human HNRNPAB were performed. The affinity constant is 1.07 nM.

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  • Featured Product
  • KD/KO Validated

HNRNPAB Recombinant antibody

HNRNPAB Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84207-1-RR
Clone No.241462B1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

hnRNP A/B, APOBEC1-binding protein 1, ABBP-1, ABBP1, ABBP 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HepG2 cells, HEK-293T cells, K-562 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inJurkat cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84207-1-RR targets HNRNPAB in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HNRNPAB fusion protein Ag35010 相同性解析による交差性が予測される生物種
Full Name heterogeneous nuclear ribonucleoprotein A/B
Calculated molecular weight36 kDa
Observed molecular weight36-42 kDa
GenBank accession numberBC036708
Gene Symbol HNRNPAB
Gene ID (NCBI) 3182
RRIDAB_3671763
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99729
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Heterogeneous nuclear ribonucleoprotein AB (HNRNPAB) is a member of the hnRNP family involved in multiple forms of transcriptional control. It plays an important role in normal biological processes and tumor development and progression. HNRNPAB acts as a direct transcriptional target of c-Myc and promotes G1/S cell cycle progression and cell proliferation via CDK4(PMID: 36657708).

Protocols

Product Specific Protocols
WB protocol for HNRNPAB antibody 84207-1-RRDownload protocol
IF protocol for HNRNPAB antibody 84207-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols