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  • Featured Product
  • KD/KO Validated

HNRNPC Polyclonal antibody

HNRNPC Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 11760-1-AP

Synonyms

SNRPC, HNRPC, hnRNP C1/C2, HNRNP, C2

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using HNRNPC Polyclonal antibody (11760-1-AP)

    WB analysis of HeLa using 11760-1-AP

    WB result of HNRNPC antibody (11760-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HNRNPC transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using HNRNPC Polyclonal antibody (11760-1-AP)

    WB analysis using 11760-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11760-1-AP (HNRNPC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using HNRNPC Polyclonal antibody (11760-1-AP)

    WB analysis of K-562 using 11760-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 11760-1-AP (HNRNPC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using HNRNPC Polyclonal antibody (11760-1-AP)

    WB analysis of human brain using 11760-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11760-1-AP (HNRNPC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using HNRNPC Polyclonal antibody (11760-1-AP)

    IP experiment of HeLa using 11760-1-AP

    IP result of anti-HNRNPC (IP:11760-1-AP, 4ug; Detection:11760-1-AP 1:10000) with HeLa cells lysate 1360 ug.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using HNRNPC Polyclonal antibody (11760-1-AP)

    IHC staining of human lymphoma using 11760-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 11760-1-AP (HNRNPC antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using HNRNPC Polyclonal antibody (11760-1-AP)

    IHC staining of mouse colon using 11760-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11760-1-AP (HNRNPC antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using HNRNPC Polyclonal antibody (11760-1-AP)

    IHC staining of mouse colon using 11760-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11760-1-AP (HNRNPC antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HNRNPC Polyclonal antibody (11760-1-AP)

    IF Staining of HeLa using 11760-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPC antibody (11760-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inHeLa cells, human brain tissue, K-562 cells, HepG2 cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse colon tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11760-1-AP targets HNRNPC in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HNRNPC fusion protein Ag2356 相同性解析による交差性が予測される生物種
Full Name heterogeneous nuclear ribonucleoprotein C (C1/C2)
Calculated molecular weight 32 kDa
Observed molecular weight 32 kDa, 36-40 kDa
GenBank accession numberBC003394
Gene symbol HNRNPC
Gene ID (NCBI) 3183
RRIDAB_2117500
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HNRNPC, also named as Heterogeneous nuclear ribonucleoproteins C1/C2, is a 306 amino acid protein, which contains 1 RRM (RNA recognition motif) domain and belongs to the RRM HNRPC family. HNRNPC localizes in the nucleus. It binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. HNRNPC may play a role in the early steps of spliceosome assembly and pre-mRNA splicing. HNRNPC can act as a tetramer and is involved in the assembly of 40S hnRNP particles and plays a role with CSDE1 in internal initiation of translation during mitosis.

Protocols

Product Specific Protocols
WB protocol for HNRNPC antibody 11760-1-APDownload protocol
IHC protocol for HNRNPC antibody 11760-1-APDownload protocol
IF protocol for HNRNPC antibody 11760-1-APDownload protocol
IP protocol for HNRNPC antibody 11760-1-APDownload protocol
FC protocol for HNRNPC antibody 11760-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

METTL3 regulates heterochromatin in mouse embryonic stem cells.

Authors - Wenqi Xu
View Article
humanWB

Cell Metab

NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.

Authors - Mi Kyung Park
View Article
humanWB

Nat Struct Mol Biol

Alternative polyadenylation by sequential activation of distal and proximal PolyA sites.

Authors - Peng Tang
View Article
humanWB

Adv Sci (Weinh)

m6A-Modified circTET2 Interacting with HNRNPC Regulates Fatty Acid Oxidation to Promote the Proliferation of Chronic Lymphocytic Leukemia

Authors - Zijuan Wu
  • KD Validated
View Article
humanWB

Nucleic Acids Res

Sequence-dependent recruitment of SRSF1 and SRSF7 to intronless lncRNA NKILA promotes nuclear export via the TREX/TAP pathway.

Authors - Misbah Khan
View Article
humanWB

Clin Transl Med

LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing

Authors - Qi Wen Chen
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Tatyana (Verified Customer) (12-04-2023)

Works very well in ICC (nuclear staining) but extra bands in WB

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human / HeLa cells, SH-SY5Y cells
HNRNPC Antibody Western Blot,Immunofluorescence validation (1:1000 dilution) in Human / HeLa cells, SH-SY5Y cells (Cat no:11760-1-AP)