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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HNRNPC Monoclonal antibody (68447-1-Ig)

    WB analysis using 68447-1-Ig (same clone as 68447-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68447-1-Ig (HNRNPC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68447-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using HNRNPC Monoclonal antibody (68447-1-Ig)

    IP experiment of HeLa using 68447-1-Ig (same clone as 68447-1-PBS)

    IP result of anti-HNRNPC (IP:68447-1-Ig, 4ug; Detection:68447-1-Ig 1:4000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 68447-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HNRNPC Monoclonal antibody (68447-1-Ig)

    IF Staining of HeLa using 68447-1-Ig (same clone as 68447-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPC antibody (68447-1-Ig, Clone: 2G8G4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68447-1-PBS in a different storage buffer formulation.

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HNRNPC Monoclonal antibody, PBS Only

HNRNPC Monoclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 68447-1-PBS
Clone No.2G8G4

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse , rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

C1, C2, HNRNP, hnRNP C1/C2, HNRNPC, HNRPC, SNRPC

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68447-1-PBS targets HNRNPC in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, mouse , rat samples.

Tested Reactivity Human, mouse , rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HNRNPC fusion protein Ag2356 相同性解析による交差性が予測される生物種
Full Name heterogeneous nuclear ribonucleoprotein C (C1/C2)
Calculated molecular weight 32 kDa
Observed molecular weight 36-40 kDa
GenBank accession numberBC003394
Gene Symbol HNRNPC
Gene ID (NCBI) 3183
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP07910
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HNRNPC, also named as Heterogeneous nuclear ribonucleoproteins C1/C2, is a 306 amino acid protein, which contains 1 RRM (RNA recognition motif) domain and belongs to the RRM HNRPC family. HNRNPC localizes in the nucleus. It binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. HNRNPC may play a role in the early steps of spliceosome assembly and pre-mRNA splicing. HNRNPC can act as a tetramer and is involved in the assembly of 40S hnRNP particles and plays a role with CSDE1 in internal initiation of translation during mitosis.