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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HNRNPC Recombinant antibody

HNRNPC Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

4F16

Cat no : 82798-3-RR

Synonyms

HNRPC, hnRNP C1/C2, HNRNP, C2, C1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HNRNPC Recombinant antibody (82798-3-RR)

    WB analysis using 82798-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82798-3-RR (HNRNPC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using HNRNPC Recombinant antibody (82798-3-RR)

    IHC staining of human stomach using 82798-3-RR

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82798-3-RR (HNRNPC antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using HNRNPC Recombinant antibody (82798-3-RR)

    IHC staining of human stomach using 82798-3-RR

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82798-3-RR (HNRNPC antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using HNRNPC Recombinant antibody (82798-3-RR)

    IHC staining of mouse colon using 82798-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82798-3-RR (HNRNPC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using HNRNPC Recombinant antibody (82798-3-RR)

    IHC staining of mouse colon using 82798-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82798-3-RR (HNRNPC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HNRNPC Recombinant antibody (82798-3-RR)

    IF Staining of HeLa using 82798-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPC antibody (82798-3-RR, Clone: 4F16 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). CoraLite® Plus 488-Phalloidin (green).

  • Affinity and Kinetic Characterization of 82798-3-RR

    Affinity and Kinetic Characterization of 82798-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82798-3-RR against Human HNRNPC were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, Jurkat cells, NIH/3T3 cells, HSC-T6 cells
Positive IHC detected inhuman stomach tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82798-3-RR targets HNRNPC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HNRNPC fusion protein Ag2356 相同性解析による交差性が予測される生物種
Full Name heterogeneous nuclear ribonucleoprotein C (C1/C2)
Calculated molecular weight 32 kDa
Observed molecular weight 36-40 kDa
GenBank accession numberBC003394
Gene symbol HNRNPC
Gene ID (NCBI) 3183
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HNRNPC antibody 82798-3-RRDownload protocol
IHC protocol for HNRNPC antibody 82798-3-RRDownload protocol
IF protocol for HNRNPC antibody 82798-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols