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HNRNPD Polyclonal antibody

Name: HNRNPD Polyclonal antibody
Brand: Proteintech
SKU: 12770-1-AP

HNRNPD Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12770-1-AP

Synonyms

AUF1, HNRPD, hnRNPD0, hnRNP D0, AU-rich element RNA-binding protein 1

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 12770-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12770-1-AP (HNRNPD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 12770-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 12770-1-AP (HNRNPD Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 12770-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 12770-1-AP (HNRNPD Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 12770-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 12770-1-AP (HNRNPD Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12770-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12770-1-AP (HNRNPD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12770-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12770-1-AP (HNRNPD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human cervical cancer using 12770-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12770-1-AP (HNRNPD antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 12770-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12770-1-AP (HNRNPD antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human normal colon using 12770-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12770-1-AP (HNRNPD antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human normal colon using 12770-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12770-1-AP (HNRNPD antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 12770-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12770-1-AP (HNRNPD antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 12770-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12770-1-AP (HNRNPD antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 12770-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer slide using 12770-1-AP (HNRNPD Antibody) at dilution of 1:50.
IHC staining of human placenta using 12770-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta slide using 12770-1-AP (HNRNPD Antibody) at dilution of 1:50.
IF Staining of HepG2 using 12770-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNRNPD antibody (12770-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Cat No. 12770-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, Jurkat cells, mouse liver tissue
Positive IHC detected in	human cervical cancer tissue, human normal colon, human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:200-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 16 publications below
IHC	See 5 publications below
IF	See 3 publications below

Product Information

12770-1-AP targets HNRNPD in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HNRNPD fusion protein Ag3458 相同性解析による交差性が予測される生物種
Full Name	heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa)
Calculated molecular weight	355 aa, 38 kDa
Observed molecular weight	38-45 kDa
GenBank accession number	BC026015
Gene symbol	HNRNPD
Gene ID (NCBI)	3184
RRID	AB_2117318
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPD, also known as AUF1, belongs to the family of AU-binding proteins (AU-BPs) that regulate the cellular half-lives of many mRNAs by directly interacting with an AU-rich element (ARE) located in their 3′ untranslated region [PMID:8578590,12704645]. AUF1 has four isoforms produced by alternative splicing of a single transcript: p37, p40, p42, and p45 (PMID:9521873).

Protocols

Product Specific Protocols
WB protocol for HNRNPD antibody 12770-1-AP	Download protocol
IHC protocol for HNRNPD antibody 12770-1-AP	Download protocol
IF protocol for HNRNPD antibody 12770-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Acetylation of Cytidine in mRNA Promotes Translation Efficiency. Authors - Daniel Arango View Article
human	WB	Clin Transl Med LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing Authors - Qi Wen Chen View Article
human	WB	Dev Cell Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia. Authors - Yanni Ma View Article
human	IHC	Brain Sequestration of multiple RNA recognition motif-containing proteins by C9orf72 repeat expansions. Authors - Johnathan Cooper-Knock View Article
human	IHC	Acta Neuropathol Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations. Authors - Neumann Manuela M View Article
human	WB	EMBO Rep Enzymatic degradation of RNA causes widespread protein aggregation in cell and tissue lysates. Authors - Johan Aarum View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HNRNPD Polyclonal antibody

HNRNPD Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 12770-1-AP

WB analysis of mouse liver using 12770-1-AP

WB analysis of Jurkat using 12770-1-AP

WB analysis of Jurkat using 12770-1-AP

WB analysis of HeLa using 12770-1-AP

WB analysis of HeLa using 12770-1-AP

IHC staining of human cervical cancer using 12770-1-AP

IHC staining of human cervical cancer using 12770-1-AP

IHC staining of human normal colon using 12770-1-AP

IHC staining of human normal colon using 12770-1-AP

IHC staining of human cervical cancer using 12770-1-AP

IHC staining of human cervical cancer using 12770-1-AP

IHC staining of human cervical cancer using 12770-1-AP

IHC staining of human placenta using 12770-1-AP

IF Staining of HepG2 using 12770-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Acetylation of Cytidine in mRNA Promotes Translation Efficiency.

LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing

Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.

Sequestration of multiple RNA recognition motif-containing proteins by C9orf72 repeat expansions.

Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations.

Enzymatic degradation of RNA causes widespread protein aggregation in cell and tissue lysates.