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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HNRNPD Monoclonal antibody

HNRNPD Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2G9E6

Cat no : 68236-1-Ig

Synonyms

AUF1, AUF1A, hnRNP D0, HNRNPD, hnRNPD0, HNRPD, P37

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HNRNPD Monoclonal antibody (68236-1-Ig)

    WB analysis using 68236-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68236-1-Ig (HNRNPD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using HNRNPD Monoclonal antibody (68236-1-Ig)

    IHC staining of human cervical cancer using 68236-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68236-1-Ig (HNRNPD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using HNRNPD Monoclonal antibody (68236-1-Ig)

    IHC staining of human cervical cancer using 68236-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68236-1-Ig (HNRNPD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HNRNPD Monoclonal antibody (68236-1-Ig)

    IF Staining of HepG2 using 68236-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNRNPD antibody (68236-1-Ig, Clone: 2G9E6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inA431 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68236-1-Ig targets HNRNPD in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Reactivity Human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HNRNPD fusion protein Ag3458 相同性解析による交差性が予測される生物種
Full Name heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa)
Calculated molecular weight 355 aa, 38 kDa
Observed molecular weight38 kDa
GenBank accession numberBC026015
Gene symbol HNRNPD
Gene ID (NCBI) 3184
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPD, also known as AUF1, belongs to the family of AU-binding proteins (AU-BPs) that regulate the cellular half-lives of many mRNAs by directly interacting with an AU-rich element (ARE) located in their 3′ untranslated region (PMID:8578590,12704645). AUF1 has four isoforms produced by alternative splicing of a single transcript: p37, p40, p42, and p45 (PMID:9521873).

Protocols

Product Specific Protocols
WB protocol for HNRNPD antibody 68236-1-IgDownload protocol
IHC protocol for HNRNPD antibody 68236-1-IgDownload protocol
IF protocol for HNRNPD antibody 68236-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols