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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HNRNPF Monoclonal antibody (67701-1-Ig)

    WB analysis using 67701-1-Ig (same clone as 67701-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67701-1-Ig (HNRNPF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67701-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using HNRNPF Monoclonal antibody (67701-1-Ig)

    WB analysis using 67701-1-Ig (same clone as 67701-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67701-1-Ig (HNRNPF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67701-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HNRNPF Monoclonal antibody (67701-1-Ig)

    IF Staining of HepG2 using 67701-1-Ig (same clone as 67701-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNRNPF antibody (67701-1-Ig, Clone: 2E7A4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67701-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

HNRNPF Monoclonal antibody, PBS Only

HNRNPF Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67701-1-PBS
Clone No.2E7A4

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

hnRNP F, HNRNPF, HNRPF, mcs94 1, Nucleolin like protein mcs94 1, OK/SW cl.23

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67701-1-PBS targets HNRNPF in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HNRNPF fusion protein Ag7128 相同性解析による交差性が予測される生物種
Full Name heterogeneous nuclear ribonucleoprotein F
Calculated molecular weight 46 kDa
Observed molecular weight46 kDa
GenBank accession numberBC001432
Gene Symbol HNRNPF
Gene ID (NCBI) 3185
RRIDAB_2882893
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP52597
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

HNRNPF, also named as Heterogeneous nuclear ribonucleoprotein F,is a 415 amino acid portein, which contains 3 RRM (RNA recognition motif) domains. HNRNPF is expressed ubiquitously in various tissues and localizes in the nucleus. HNRNPF is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPF plays a role in the regulation of alternative splicing events.