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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HNRNPK Monoclonal antibody (67708-1-Ig)

    WB analysis using 67708-1-Ig (same clone as 67708-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67708-1-Ig (HNRNPK antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67708-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using HNRNPK Monoclonal antibody (67708-1-Ig)

    WB analysis using 67708-1-Ig (same clone as 67708-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67708-1-Ig (HNRNPK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67708-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HNRNPK Monoclonal antibody (67708-1-Ig)

    IHC staining of human colon cancer using 67708-1-Ig (same clone as 67708-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67708-1-Ig (HNRNPK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67708-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HNRNPK Monoclonal antibody (67708-1-Ig)

    IHC staining of human colon cancer using 67708-1-Ig (same clone as 67708-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67708-1-Ig (HNRNPK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67708-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HNRNPK Monoclonal antibody (67708-1-Ig)

    IHC staining of human breast cancer using 67708-1-Ig (same clone as 67708-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67708-1-Ig (HNRNPK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67708-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HNRNPK Monoclonal antibody (67708-1-Ig)

    IHC staining of human breast cancer using 67708-1-Ig (same clone as 67708-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67708-1-Ig (HNRNPK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67708-1-PBS in a different storage buffer formulation.

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HNRNPK Monoclonal antibody, PBS Only

HNRNPK Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67708-1-PBS
Clone No.1A10C8

Host / Isotype

Mouse / IgM

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CSBP, FLJ41122, hnRNP K, HNRNPK, HNRPK, TUNP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67708-1-PBS targets HNRNPK in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen HNRNPK fusion protein Ag30093 相同性解析による交差性が予測される生物種
Full Name heterogeneous nuclear ribonucleoprotein K
Calculated molecular weight 463 aa, 51 kDa
Observed molecular weight60 kDa
GenBank accession numberBC014980
Gene Symbol HNRNPK
Gene ID (NCBI) 3190
RRIDAB_2882899
Conjugate Unconjugated
Form Liquid
Purification MethodThiophilic affinity chromatograph
UNIPROT IDP61978
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

HNRNPK belongs to the heterogeneous nuclear ribonucleoprotein family of 20 proteins that participate in a wide range of key cellular functions involving many of the pathways implicated, disrupted or dysregulated in tumor development and progression. It is also a conserved pre-mRNA-binding protein that is involved in multiple processes of gene expression, including chromatin remodeling, transcription, and mRNA splicing, translation, and stability