- Featured Product
- KD/KO Validated
HNRNPL Polyclonal antibody
HNRNPL Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (3)
Applications
WB, IHC, IP, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 18354-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | BxPC-3 cells, mouse liver tissue, PC-3 cells, RAW 264.7 cells |
| Positive IP detected in | BxPC-3 cells |
| Positive IHC detected in | human stomach cancer tissue, human kidney tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IHC | See 1 publications below |
| ChIP | See 1 publications below |
Product Information
18354-1-AP targets HNRNPL in WB, IHC, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, pig, bovine, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HNRNPL fusion protein Ag13211 相同性解析による交差性が予測される生物種 |
| Full Name | heterogeneous nuclear ribonucleoprotein L |
| Calculated molecular weight | 572 aa, 62 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC069184 |
| Gene symbol | HNRNPL |
| Gene ID (NCBI) | 3191 |
| RRID | AB_2878537 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Heterogeneous nuclear ribonucleoprotein L (hnRNP L) is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. It is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. The two isoforms of hnRNP L are generated by alternative splicing. The human hnRNPL gene encodes a protein that is localized in the nucleoplasm and cytoplasm. This antibody may recognize both isoforms of the hnRNP L protein.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HNRNPL antibody 18354-1-AP | Download protocol |
| IHC protocol for HNRNPL antibody 18354-1-AP | Download protocol |
| IP protocol for HNRNPL antibody 18354-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
RNA Biol CFIm25 regulates human stem cell function independently of its role in mRNA alternative polyadenylation. | ||
J Proteome Res C1QBP Negatively Regulates the Activation of Oncoprotein YBX1 in the Renal Cell Carcinoma as Revealed by Interactomics Analysis. | ||
J Virol Heterogeneous Nuclear Ribonucleoprotein L Negatively Regulates Foot-and-Mouth Disease Virus Replication through Inhibition of Viral RNA Synthesis by Interacting with the Internal Ribosome Entry Site in the 5' Untranslated Region.
| ||
DNA Cell Biol HNRNPL Is Identified and Validated as a Prognostic Biomarker Associated with Microsatellite Instability in Human Gastric Cancer. |
