Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, HeLa cells |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
| IHC | See 2 publications below |
Product Information
15018-1-AP targets HNRNPR in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HNRNPR fusion protein Ag7028 相同性解析による交差性が予測される生物種 |
| Full Name | heterogeneous nuclear ribonucleoprotein R |
| Calculated molecular weight | 71 kDa |
| Observed molecular weight | 65-82 kDa |
| GenBank accession number | BC001449 |
| Gene Symbol | HNRNPR |
| Gene ID (NCBI) | 10236 |
| RRID | AB_2120506 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43390 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HNRNPR belongs to Heterogeneous nuclear ribonucleoproteins (hnRNPs), which are among the most abundant proteins in the eukaryotic nucleus and they play important roles in processing of precursor mRNA. hnRNP proteins have a modular structure consisting of one or more RNA binding domains and at least another auxiliary domain, which likely mediates protein-protein interactions but may also contribute to nucleic acid binding. The calcualted molecular weight of HNRNPR is 71 kDa, but the migration behavior of the 82 kDa translation product was indistinguishable from that of HNRNPR. (PMID: 9421497)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HNRNPR antibody 15018-1-AP | Download protocol |
| IHC protocol for HNRNPR antibody 15018-1-AP | Download protocol |
| IF protocol for HNRNPR antibody 15018-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Immunol HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins.
| ||
Neurosci Lett hnRNPM deficiency leads to cognitive deficits via disrupting synaptic plasticity. | ||
Front Oncol The hnRNPK/A1/R/U Complex Regulates Gene Transcription and Translation and is a Favorable Prognostic Biomarker for Human Colorectal Adenocarcinoma. | ||
iScience Increased LDL receptor by SREBP2 or SREBP2-induced lncRNA LDLR-AS promotes triglyceride accumulation in fish
| ||
J Transl Med High expression of HNRNPR in ESCA combined with 18F-FDG PET/CT metabolic parameters are novel biomarkers for preoperative diagnosis of ESCA | ||
Eur J Pharm Sci Novel facile thermosensitive hydrogel as sustained and controllable gene release vehicle for breast cancer treatment. |