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HNRNPU Polyclonal antibody

Name: HNRNPU Polyclonal antibody
Brand: Proteintech
SKU: 16365-1-AP

HNRNPU Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 16365-1-AP

Synonyms

hnRNP U, HNRNPU, HNRPU, p120, pp120, SAF A, SAFA, Scaffold attachment factor A, U21.1

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis of HEK-293 using 16365-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 16365-1-AP (HNRNPU antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16365-1-AP
WB result of HNRNPU antibody (16365-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HNRNPU transfected HeLa cells.
WB analysis of HeLa using 16365-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16365-1-AP (HNRNPU antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 16365-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 16365-1-AP (HNRNPU antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 16365-1-AP
IP result of anti-HNRNPU (IP:16365-1-AP, 4ug; Detection:16365-1-AP 1:8000) with HeLa cells lysate 2000 ug.
IHC staining of mouse kidney using 16365-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16365-1-AP (HNRNPU antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 16365-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16365-1-AP (HNRNPU antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 16365-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNRNPU antibody (16365-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
FC experiment of HepG2 using 16365-1-AP
1X10^6 HepG2 cells were stained with 0.2ug HNRNPU antibody (16365-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Cat No. 16365-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, Jurkat cells
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells
Positive FC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)	FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below
IHC	See 1 publications below
IF	See 2 publications below
IP	See 1 publications below

Product Information

16365-1-AP targets HNRNPU in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HNRNPU fusion protein Ag9151 相同性解析による交差性が予測される生物種
Full Name	heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A)
Calculated molecular weight	91 kDa
Observed molecular weight	120 kDa
GenBank accession number	BC003367
Gene symbol	HNRNPU
Gene ID (NCBI)	3192
RRID	AB_10596481
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HNRNPU, also named as HNRPU, SAFA, and U21.1, is a 825 amino acid protein, which is extensively phosphorylated. HNRNPU as a component of ribonucleosomes localized in cytoplasmic mRNP granules containing untranslated mRNAs. HNRNPU is a component of the CRD-mediated complex that promotes MYC mRNA stabilization. HNRNPU Plays a role in the circadian regulation of the core clock component ARNTL/BMAL1 transcription. The calculated molecular weight of HNRNPU is 90 kDa, but the 120 kDa HNRNPU protein is an abundant nucleoplasmic phosphoprotein that can be crosslinked to pre-mRNA in intact cells by UV light (PMID: 1628625).

Protocols

Product Specific Protocols
WB protocol for HNRNPU antibody 16365-1-AP	Download protocol
IHC protocol for HNRNPU antibody 16365-1-AP	Download protocol
IF protocol for HNRNPU antibody 16365-1-AP	Download protocol
IP protocol for HNRNPU antibody 16365-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Dev Cell Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia. Authors - Yanni Ma View Article
human	IF	Cancer Res DIS3L2 promotes progression of hepatocellular carcinoma via hnRNP U-mediated alternative splicing. Authors - Songge Xing View Article
human	IP,IF	mSystems Proteomic Profiling Identifies Kaposi's Sarcoma-Associated Herpesvirus (KSHV)-Encoded LANASIM-Associated Proteins in Hypoxia. Authors - Xiaoqing Liu View Article
		Cell Prolif Protein phosphatase 4 plays dual roles during cell proliferation. Authors - Xiuqing Huang View Article
human	WB	J Exp Clin Cancer Res A novel protein encoded by circHNRNPU promotes multiple myeloma progression by regulating the bone marrow microenvironment and alternative splicing. Authors - Xiaozhu Tang KD Validated View Article
human	IHC,WB	Front Oncol The hnRNPK/A1/R/U Complex Regulates Gene Transcription and Translation and is a Favorable Prognostic Biomarker for Human Colorectal Adenocarcinoma. Authors - Yixin Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HNRNPU Polyclonal antibody

HNRNPU Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis of HEK-293 using 16365-1-AP

WB analysis of HeLa using 16365-1-AP

WB analysis of HeLa using 16365-1-AP

WB analysis using 16365-1-AP

IP experiment of HeLa using 16365-1-AP

IHC staining of mouse kidney using 16365-1-AP

IHC staining of mouse kidney using 16365-1-AP

IF Staining of HepG2 using 16365-1-AP

FC experiment of HepG2 using 16365-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.

DIS3L2 promotes progression of hepatocellular carcinoma via hnRNP U-mediated alternative splicing.

Proteomic Profiling Identifies Kaposi's Sarcoma-Associated Herpesvirus (KSHV)-Encoded LANASIM-Associated Proteins in Hypoxia.

Protein phosphatase 4 plays dual roles during cell proliferation.

A novel protein encoded by circHNRNPU promotes multiple myeloma progression by regulating the bone marrow microenvironment and alternative splicing.

The hnRNPK/A1/R/U Complex Regulates Gene Transcription and Translation and is a Favorable Prognostic Biomarker for Human Colorectal Adenocarcinoma.