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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HNRNPUL2-Specific Polyclonal antibody (20143-1-AP)

    WB analysis using 20143-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20143-1-AP (HNRNPUL2-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using HNRNPUL2-Specific Polyclonal antibody (20143-1-AP)

    IF Staining of MCF-7 using 20143-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HNRNPUL2-Specific antibody (20143-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using HNRNPUL2-Specific Polyclonal antibody (20143-1-AP)

    IF Staining of MCF-7 using 20143-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HNRNPUL2-Specific antibody (20143-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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HNRNPUL2-Specific Polyclonal antibody

HNRNPUL2-Specific Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 20143-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

HNRNPUL2, Heterogeneous nuclear ribonucleoprotein U-like protein 2, HNRNP, HNRPUL2, SAF A2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20143-1-AP targets HNRNPUL2-Specific in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name heterogeneous nuclear ribonucleoprotein U-like 2
Calculated molecular weight 85 kDa
Observed molecular weight 110-120 kDa
GenBank accession numberNM_001079559
Gene Symbol HNRNPUL2
Gene ID (NCBI) 221092
RRIDAB_2878646
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ1KMD3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HNRNPUL2 also named as SAF-A2. This antibody is specific to HNRNPUL2.

Protocols

Product Specific Protocols
WB protocol for HNRNPUL2-Specific antibody 20143-1-APDownload protocol
IF protocol for HNRNPUL2-Specific antibody 20143-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols