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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HNRPDL Polyclonal antibody

HNRPDL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10660-2-AP

Synonyms

hnRNP D-like, hnRNP DL, hnRNP D like, HNRNP, Heterogeneous nuclear ribonucleoprotein D-like

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HNRPDL Polyclonal antibody (10660-2-AP)

    WB analysis using 10660-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10660-2-AP (HNRPDL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using HNRPDL Polyclonal antibody (10660-2-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 10660-2-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10660-2-AP (HNRPDL antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHL-60 cells, HeLa cells, K-562 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10660-2-AP targets HNRPDL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HNRPDL fusion protein Ag1046 相同性解析による交差性が予測される生物種
Full Name heterogeneous nuclear ribonucleoprotein D-like
Calculated molecular weight 46 aa, 4 kDa
Observed molecular weight 38-40 kDa
GenBank accession numberBC007392
Gene symbol HNRPDL
Gene ID (NCBI) 9987
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HNRPDL antibody 10660-2-APDownload protocol
IHC protocol for HNRPDL antibody 10660-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cell Biochem Funct

Overexpression of JKTBP1 induces androgen-independent LNCaP cell proliferation through activation of epidermal growth factor-receptor (EGF-R).

Authors - Yong-Yang Wu
View Article