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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HNRPDL Recombinant antibody

HNRPDL Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230415F9

Cat no : 83129-5-RR

Synonyms

hnRNP DL, hnRNP D like, HNRNP, Heterogeneous nuclear ribonucleoprotein D-like, AU-rich element RNA-binding factor

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HNRPDL Recombinant antibody (83129-5-RR)

    WB analysis using 83129-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83129-5-RR (HNRPDL antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using HNRPDL Recombinant antibody (83129-5-RR)

    IF Staining of U-251 using 83129-5-RR

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using HNRPDL antibody (83129-5-RR, Clone: 230415F9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using HNRPDL Recombinant antibody (83129-5-RR)

    FC experiment of HeLa using 83129-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HNRPDL Recombinant antibody (83129-5-RR, Clone:230415F9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Tested Applications

Positive WB detected inJurkat cells, Raji cells, PC-3 cells, fetal human brain tissue, mouse retina tissue
Positive IF/ICC detected inU-251 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83129-5-RR is HNRPDL Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HNRPDL fusion protein Ag1046 相同性解析による交差性が予測される生物種
Full Name heterogeneous nuclear ribonucleoprotein D-like
Calculated molecular weight 46 aa, 4 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC007392
Gene symbol HNRPDL
Gene ID (NCBI) 9987
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HNRPDL antibody 83129-5-RRDownload protocol
IF protocol for HNRPDL antibody 83129-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols