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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

HO-1 Recombinant antibody, PBS Only

HO-1 Recombinant Antibody for WB, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2M6

Cat no : 81281-1-PBS

Synonyms

bK286B10, heme oxygenase (decycling) 1, Heme oxygenase 1, HMOX1, HO, HO 1, HO1, HSP32

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HO-1 Recombinant antibody (81281-1-RR)

    WB analysis using 81281-1-RR (same clone as 81281-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81281-1-RR (HO-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81281-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using HO-1 Recombinant antibody (81281-1-RR)

    WB analysis of HeLa using 81281-1-RR (same clone as 81281-1-PBS)

    WB result of HO-1 antibody (81281-1-RR; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HO-1 transfected HeLa cells. This data was developed using the same antibody clone with 81281-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using HO-1 Recombinant antibody (81281-1-RR)

    IP experiment of HeLa using 81281-1-RR (same clone as 81281-1-PBS)

    IP result of anti-HO-1 (IP:81281-1-RR, 4ug; Detection:81281-1-RR 1:8000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 81281-1-PBS in a different storage buffer formulation.

  • Affinity experiments of 81281-1-RR

    Affinity experiments of 81281-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81281-1-RR against Human HO-1 were performed. The affinity constant is below 1 pM.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81281-1-PBS targets HO-1 in WB, IP, Indirect ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HO-1 fusion protein Ag1190 相同性解析による交差性が予測される生物種
Full Name heme oxygenase (decycling) 1
Calculated molecular weight 33 kDa
Observed molecular weight33 kDa
GenBank accession numberBC001491
Gene symbol HMOX1
Gene ID (NCBI) 3162
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663).