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HOMER1 Polyclonal antibody
HOMER1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
Conjugate
Unconjugated
Cat no : 12433-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | rat brain tissue, human brain tissue, human/mouse brain |
Positive IP detected in | fetal human brain tissue |
Positive IHC detected in | human gliomas tissue, mouse brain tissue, rat brain tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | rat brain tissue |
Positive IF/ICC detected in | PC-12 cells, MCF-7 cells |
Positive FC (Intra) detected in | SH-SY5Y cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:5000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 10 publications below |
IHC | See 2 publications below |
IF | See 6 publications below |
Product Information
12433-1-AP targets HOMER1 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | mouse, rat, zebra finches |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | HOMER1 fusion protein Ag3109 相同性解析による交差性が予測される生物種 |
Full Name | homer homolog 1 (Drosophila) |
Calculated molecular weight | 40 kDa |
Observed molecular weight | 46-48 kDa |
GenBank accession number | BC015502 |
Gene symbol | HOMER1 |
Gene ID (NCBI) | 9456 |
RRID | AB_2295573 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Human HOMER1, also named as SYN47, belongs to the Homer family. Human HOMER1 has some isoforms: HOMER1a, HOMER1b, HOMER1c, HOMER1e and HOMER1h. Mouse Homer1 also named as Vesl-1 and it has some isoforms: Vesl-1l, Vesl-1m, Vesl-1s, Homer1b, and Homer1d. HOMER1 is a postsynaptic density scaffolding protein. It is a neuronal immediate-early gene (IEG) that is enriched at excitatory synapses and binds mGluRs. In WB, the MW of HOMER1 is about 46-50kd, 40-43kd and 20-26kd.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for HOMER1 antibody 12433-1-AP | Download protocol |
IHC protocol for HOMER1 antibody 12433-1-AP | Download protocol |
IF protocol for HOMER1 antibody 12433-1-AP | Download protocol |
IP protocol for HOMER1 antibody 12433-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Psychiatry A recurrent SHANK1 mutation implicated in autism spectrum disorder causes autistic-like core behaviors in mice via downregulation of mGluR1-IP3R1-calcium signaling. | ||
PLoS Genet Cell fate specification in the lingual epithelium is controlled by antagonistic activities of Sonic hedgehog and retinoic acid. | ||
Aging (Albany NY) Liraglutide preconditioning attenuates myocardial ischemia/ reperfusion injury via homer1 activation.
| ||
CNS Neurosci Ther Acid-sensing Ion Channels Activation and Hypoxia Upregulate Homer1a Expression. | ||
Cell Signal Tissue kallikrein protects neurons from hypoxia/reoxygenation-induced cell injury through Homer1b/c. |