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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HOMER2 Polyclonal antibody

HOMER2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11143-1-AP

Synonyms

Homer protein homolog 2, homer homolog 2 (Drosophila), HOMER 2, Cupidin, CPD

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HOMER2 Polyclonal antibody (11143-1-AP)

    WB analysis using 11143-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11143-1-AP (HOMER2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using HOMER2 Polyclonal antibody (11143-1-AP)

    WB analysis using 11143-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11143-1-AP (HOMER2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using HOMER2 Polyclonal antibody (11143-1-AP)

    IHC staining of mouse cerebellum using 11143-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 11143-1-AP (HOMER2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using HOMER2 Polyclonal antibody (11143-1-AP)

    IHC staining of mouse cerebellum using 11143-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 11143-1-AP (HOMER2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using HOMER2 Polyclonal antibody (11143-1-AP)

    IF Staining of U2OS using 11143-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using HOMER2 antibody (11143-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, HEK-293T cells, MCF-7 cells, rat kidney tissue, LnCaP cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11143-1-AP targets HOMER2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HOMER2 fusion protein Ag1606 相同性解析による交差性が予測される生物種
Full Name homer homolog 2 (Drosophila)
Calculated molecular weight 41 kDa
Observed molecular weight41 kDa
GenBank accession numberBC012109
Gene symbol HOMER2
Gene ID (NCBI) 9455
RRIDAB_2877747
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HOMER2 (Homer Scaffold Protein 2), a postsynaptic density scaffolding protein, is essential for normal hearing in humans and mice(PMID: 25816005). HOMER2 negatively regulates T cell activation by inhibiting the calcineurin-NFAT pathway(Uniprot). HOMER2 belongs to the homer family of dendritic proteins which are immediate early gene products who bind to group 1 metabotropic glutamate receptors (mGluRs), proteins associated to triggering intracellular calcium release. Additionally, HOMER2 connects mGluRs to the inositol triphosphate receptors (IP3R).

Protocols

Product Specific Protocols
WB protocol for HOMER2 antibody 11143-1-APDownload protocol
IHC protocol for HOMER2 antibody 11143-1-APDownload protocol
IF protocol for HOMER2 antibody 11143-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Psychiatry

A recurrent SHANK1 mutation implicated in autism spectrum disorder causes autistic-like core behaviors in mice via downregulation of mGluR1-IP3R1-calcium signaling.

Authors - Yue Qin
View Article