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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HOMER2 Monoclonal antibody

HOMER2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G5G1

Cat no : 67377-1-Ig

Synonyms

ACPD, CPD, Cupidin, HOMER 2, homer homolog 2 (Drosophila), Homer protein homolog 2, HOMER2, HOMER2A, HOMER2B, Vesl 2

Validation Data Gallery

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  • Western Blot (WB) analysis of LNCaP cells using HOMER2 Monoclonal antibody (67377-1-Ig)

    WB analysis of LNCaP using 67377-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67377-1-Ig (HOMER2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using HOMER2 Monoclonal antibody (67377-1-Ig)

    WB analysis of HEK-293 using 67377-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67377-1-Ig (HOMER2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using HOMER2 Monoclonal antibody (67377-1-Ig)

    IHC staining of human endometrial cancer using 67377-1-Ig

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 67377-1-Ig (HOMER2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using HOMER2 Monoclonal antibody (67377-1-Ig)

    IHC staining of human endometrial cancer using 67377-1-Ig

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 67377-1-Ig (HOMER2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inLNCaP cells, HEK-293 cells
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67377-1-Ig targets HOMER2 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen HOMER2 fusion protein Ag25177 相同性解析による交差性が予測される生物種
Full Name homer homolog 2 (Drosophila)
Calculated molecular weight 41 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC012109
Gene symbol HOMER2
Gene ID (NCBI) 9455
RRIDAB_2882624
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HOMER2 antibody 67377-1-IgDownload protocol
IHC protocol for HOMER2 antibody 67377-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols