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KD/KO Validated

HOOK3 Polyclonal antibody

Name: HOOK3 Polyclonal antibody
Brand: Proteintech
SKU: 15457-1-AP

HOOK3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15457-1-AP

Synonyms

Protein Hook homolog 3, h-hook3, hHK3

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of rat liver using 15457-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 15457-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 15457-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 15457-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 15457-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 15457-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 15457-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse kidney using 15457-1-AP
IP result of anti-HOOK3 (IP:15457-1-AP, 4ug; Detection:15457-1-AP 1:500) with mouse kidney tissue lysate 4000ug.
IHC staining of human kidney using 15457-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15457-1-AP (HOOK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 15457-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15457-1-AP (HOOK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 15457-1-AP
Immunofluorescent analysis of HepG2 cells, using HOOK3 antibody 15457-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Cat No. 15457-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	rat liver tissue, HEK-293 cells, mouse kidney tissue, human liver tissue, mouse heart tissue, mouse liver tissue
Positive IP detected in	mouse kidney tissue
Positive IHC detected in	human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 11 publications below
IHC	See 2 publications below
IF	See 2 publications below
IP	See 1 publications below

Product Information

15457-1-AP targets HOOK3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HOOK3 fusion protein Ag7724 相同性解析による交差性が予測される生物種
Full Name	hook homolog 3 (Drosophila)
Calculated molecular weight	83 kDa
Observed molecular weight	83 kDa
GenBank accession number	BC056146
Gene symbol	HOOK3
Gene ID (NCBI)	84376
RRID	AB_2120828
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for HOOK3 antibody 15457-1-AP	Download protocol
IHC protocol for HOOK3 antibody 15457-1-AP	Download protocol
IF protocol for HOOK3 antibody 15457-1-AP	Download protocol
IP protocol for HOOK3 antibody 15457-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun PTPN21 and Hook3 relieve KIF1C autoinhibition and activate intracellular transport. Authors - Nida Siddiqui View Article
rat	WB	Dev Cell The Dynein Adaptor RILP Controls Neuronal Autophagosome Biogenesis, Transport, and Clearance. Authors - Noopur V Khobrekar View Article
human	WB,IP	J Cell Biol Hook3 is a scaffold for the opposite-polarity microtubule-based motors cytoplasmic dynein-1 and KIF1C. Authors - Agnieszka A Kendrick View Article
rat	WB	J Cell Biol Dynein activator Hook1 is required for trafficking of BDNF-signaling endosomes in neurons. Authors - Mara A Olenick View Article
mouse	WB	Mol Cell Proteomics Spatiotemporal Changes of the Phagosomal Proteome in Dendritic Cells in Response to LPS Stimulation Authors - Anne-Marie Pauwels View Article
human	WB	iScience Mitotic HOOK3 phosphorylation by ERK1c drives microtubule-dependent Golgi destabilization and fragmentation. Authors - Inbal Wortzel KO Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HOOK3 Polyclonal antibody

HOOK3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of rat liver using 15457-1-AP

WB analysis of mouse liver using 15457-1-AP

WB analysis of HEK-293 using 15457-1-AP

WB analysis of mouse kidney using 15457-1-AP

WB analysis of rat liver using 15457-1-AP

WB analysis of mouse heart using 15457-1-AP

WB analysis of human liver using 15457-1-AP

IP experiment of mouse kidney using 15457-1-AP

IHC staining of human kidney using 15457-1-AP

IHC staining of human kidney using 15457-1-AP

IF Staining of HepG2 using 15457-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

PTPN21 and Hook3 relieve KIF1C autoinhibition and activate intracellular transport.

The Dynein Adaptor RILP Controls Neuronal Autophagosome Biogenesis, Transport, and Clearance.

Hook3 is a scaffold for the opposite-polarity microtubule-based motors cytoplasmic dynein-1 and KIF1C.

Dynein activator Hook1 is required for trafficking of BDNF-signaling endosomes in neurons.

Spatiotemporal Changes of the Phagosomal Proteome in Dendritic Cells in Response to LPS Stimulation

Mitotic HOOK3 phosphorylation by ERK1c drives microtubule-dependent Golgi destabilization and fragmentation.