HORMAD1 Polyclonal antibody
HORMAD1 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 13917-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HUVEC cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 9 publications below |
| IF | See 20 publications below |
| CoIP | See 1 publications below |
Product Information
13917-1-AP targets HORMAD1 in WB, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HORMAD1 fusion protein Ag4914 相同性解析による交差性が予測される生物種 |
| Full Name | HORMA domain containing 1 |
| Calculated molecular weight | 45 kDa |
| Observed molecular weight | 45 kDa |
| GenBank accession number | BC047406 |
| Gene symbol | HORMAD1 |
| Gene ID (NCBI) | 84072 |
| RRID | AB_2120844 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HORMA domain-containing proteins regulate interactions between homologous chromosomes (homologs) during meiosis in a wide range of eukaryotes [PMID:21079677]. They also implicated in other processes related to crossover formation, including DSB formation, inhibition of promiscuous formation of the synaptonemal complex (SC), and the meiotic prophase checkpoint that monitors both DSB processing and SCs [PMID:19851446]. HORMAD1 first accumulates on the chromosomes during the leptotene to zygotene stages of meiotic prophase I. As germ cells progress into the pachytene stage, HORMAD1 disappears from the synapsed chromosomal regions. However, once the chromosomes desynapse during the diplotene stage, HORMAD1 again accumulates on the chromosome axis of the desynapsed homologs [PMID:19686734]. HORMAD1 exists as various isoform and the range of molecular weight of isoforms are sbout 36-45 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HORMAD1 antibody 13917-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res SCF ubiquitin E3 ligase regulates DNA double-strand breaks in early meiotic recombination. | ||
Nucleic Acids Res NAT10-mediated N4-acetylcytidine modification is required for meiosis entry and progression in male germ cells. | ||
Curr Biol Prdm9 and Meiotic Cohesin Proteins Cooperatively Promote DNA Double-Strand Break Formation in Mammalian Spermatocytes. | ||
PLoS Genet Phosphorylation of chromosome core components may serve as axis marks for the status of chromosomal events during mammalian meiosis. | ||
PLoS Genet Meiotic cohesins mediate initial loading of HORMAD1 to the chromosomes and coordinate SC formation during meiotic prophase. |
