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Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using HORMAD1 Monoclonal antibody (67091-1-Ig)

    WB analysis of human testis using 67091-1-Ig (same clone as 67091-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 67091-1-Ig (HORMAD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67091-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using HORMAD1 Monoclonal antibody (67091-1-Ig)

    WB analysis of mouse testis using 67091-1-Ig (same clone as 67091-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 67091-1-Ig (HORMAD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67091-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HORMAD1 Monoclonal antibody (67091-1-Ig)

    IHC staining of human breast cancer using 67091-1-Ig (same clone as 67091-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67091-1-Ig (HORMAD1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67091-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HORMAD1 Monoclonal antibody (67091-1-Ig)

    IHC staining of human breast cancer using 67091-1-Ig (same clone as 67091-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67091-1-Ig (HORMAD1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67091-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HORMAD1 Monoclonal antibody (67091-1-Ig)

    IF Staining of HepG2 using 67091-1-Ig (same clone as 67091-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67091-1-Ig (HORMAD1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67091-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

HORMAD1 Monoclonal antibody, PBS Only

HORMAD1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67091-1-PBS
Clone No.2D7E12

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Cancer/testis antigen 46, CT46, DKFZp434A1315, HORMA domain containing 1, HORMAD1, Newborn ovary HORMA protein, NOHMA, RP11 363I22.1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67091-1-PBS targets HORMAD1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HORMAD1 fusion protein Ag28316 相同性解析による交差性が予測される生物種
Full Name HORMA domain containing 1
Calculated molecular weight 45 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC047406
Gene Symbol HORMAD1
Gene ID (NCBI) 84072
RRIDAB_2882396
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ86X24
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HORMA domain-containing proteins regulate interactions between homologous chromosomes (homologs) during meiosis in a wide range of eukaryotes [PMID:21079677]. They also implicated in other processes related to crossover formation, including DSB formation, inhibition of promiscuous formation of the synaptonemal complex (SC), and the meiotic prophase checkpoint that monitors both DSB processing and SCs [PMID:19851446]. HORMAD1 first accumulates on the chromosomes during the leptotene to zygotene stages of meiotic prophase I. As germ cells progress into the pachytene stage, HORMAD1 disappears from the synapsed chromosomal regions. However, once the chromosomes desynapse during the diplotene stage, HORMAD1 again accumulates on the chromosome axis of the desynapsed homologs [PMID:19686734].