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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HOXA7 Monoclonal antibody (67112-1-Ig)

    WB analysis using 67112-1-Ig (same clone as 67112-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 67112-1-Ig (HOXA7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67112-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using HOXA7 Monoclonal antibody (67112-1-Ig)

    WB analysis using 67112-1-Ig (same clone as 67112-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67112-1-Ig (HOXA7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67112-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using HOXA7 Monoclonal antibody (67112-1-Ig)

    WB analysis of THP-1 using 67112-1-Ig (same clone as 67112-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67112-1-Ig (HOXA7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67112-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using HOXA7 Monoclonal antibody (67112-1-Ig)

    WB analysis of HepG2 using 67112-1-Ig (same clone as 67112-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67112-1-Ig (HOXA7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67112-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SMMC-7721 cells using HOXA7 Monoclonal antibody (67112-1-Ig)

    WB analysis of SMMC-7721 using 67112-1-Ig (same clone as 67112-1-PBS)

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 67112-1-Ig (HOXA7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67112-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-29 cells using HOXA7 Monoclonal antibody (67112-1-Ig)

    WB analysis of HT-29 using 67112-1-Ig (same clone as 67112-1-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 67112-1-Ig (HOXA7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67112-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using HOXA7 Monoclonal antibody (67112-1-Ig)

    WB analysis of HL-60 using 67112-1-Ig (same clone as 67112-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 67112-1-Ig (HOXA7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67112-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using HOXA7 Monoclonal antibody (67112-1-Ig)

    WB analysis of K-562 using 67112-1-Ig (same clone as 67112-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 67112-1-Ig (HOXA7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67112-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HOXA7 Monoclonal antibody (67112-1-Ig)

    IF Staining of HepG2 using 67112-1-Ig (same clone as 67112-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67112-1-Ig (HOXA7 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67112-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

HOXA7 Monoclonal antibody, PBS Only

HOXA7 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67112-1-PBS
Clone No.2A7H6

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

HOX1.1, HOX1, Homeobox protein Hox-A7, Homeobox protein Hox-1A, ANTP

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67112-1-PBS targets HOXA7 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HOXA7 fusion protein Ag19025 相同性解析による交差性が予測される生物種
Full Name homeobox A7
Calculated molecular weight 230 aa, 25 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC148692
Gene Symbol HOXA7
Gene ID (NCBI) 3204
RRIDAB_2882416
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP31268
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HOX genes play a fundamental role in the development of the vertebrate central nervous system, heart, axial skeleton, limbs, gut, urogenital tract and external genitalia. The homeobox gene Hoxa-1 is transcriptionally regulated by retinoic acid (RA) and encodes a transcription factor, which has been shown to play important roles in cell differentiation and embryogenesis. Hoxa-1 is also expressed in cancers, such as mammary tumors, though it is not expressed in normal gland or in precancerous mammary tissues. At embryonic stages, Hoxa-2 is expressed in the mesenchyme and epithelial cells of palate, however its expression is restricted to the tips of the growing palatal shelves. Hoxa-2 protein is predominantly expressed in the nuclei of cells in the ventral mantle region of the developing embryo. In the developing and adult mouse spinal cord, Hoxa-2 protein may contribute to dorsal-ventral patterning and/or to the specification of neuronal phenotype. Hoxa-7 functions as a potent transcriptional repressor and its action as such requires several domains, including both activator and repressor regions. Hoxa-7 is expressed in the fetal liver, lung, skeletal muscle, kidney, pancreas and placenta.