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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HOXA9 Polyclonal antibody

HOXA9 Polyclonal Antibody for IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 29978-1-AP

Synonyms

HOX1G, HOX1.7, HOX1, Homeobox protein Hox-A9, Homeobox protein Hox-1G

Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of HEK-293 cells using HOXA9 Polyclonal antibody (29978-1-AP)

    IP experiment of HEK-293 using 29978-1-AP

    IP result of anti-HOXA9 (IP:29978-1-AP, 4ug; Detection:29978-1-AP 1:800) with HEK-293 cells lysate 1480 ug.

  • Immunohistochemistry (IHC) staining of human colon tissue using HOXA9 Polyclonal antibody (29978-1-AP)

    IHC staining of human colon using 29978-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29978-1-AP (HOXA9 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29978-1-AP targets HOXA9 in IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HOXA9 fusion protein Ag32309 相同性解析による交差性が予測される生物種
Full Name homeobox A9
Calculated molecular weight 30 kDa
Observed molecular weight36 kDa
GenBank accession numberBC010023
Gene symbol HOXA9
Gene ID (NCBI) 3205
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Homeobox protein Hox-A9 (HOXA9) also known as HOX1G,a member of HOX family belonging to the HOXA cluster, is often studied in acute myeloid leukemia (AML), which is linked to proliferation, differentiation, and progenitor self-renewal maintenance. It is located in nucleoplasm and functions as a critical regulator of hematopoiesis, essential for the maintenance of hematopoietic stem cells and their differentiation into myeloid lineages (PMID: 34743404). Many post-transcriptional events including microRNAs, long non-coding RNAs, and epigenetic modification could also determine HOXA9 protein level and function (PMID: 36376832).

Protocols

Product Specific Protocols
IHC protocol for HOXA9 antibody 29978-1-APDownload protocol
IP protocol for HOXA9 antibody 29978-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols