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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using HOXB1 Polyclonal antibody (21653-1-AP)

    WB analysis of mouse brain using 21653-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21653-1-AP (HOXB1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using HOXB1 Polyclonal antibody (21653-1-AP)

    WB analysis of NIH/3T3 using 21653-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21653-1-AP (HOXB1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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HOXB1 Polyclonal antibody

HOXB1 Polyclonal Antibody for WB, ELISA
Cat No. 21653-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

homeobox B1, Homeobox protein Hox 2I, Homeobox protein Hox B1, Hox 2.9, HOX2, HOX2I, HOXB1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inmouse brain tissue, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21653-1-AP targets HOXB1 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HOXB1 fusion protein Ag16366 相同性解析による交差性が予測される生物種
Full Name homeobox B1
Calculated molecular weight 235 aa, 26 kDa
Observed molecular weight 41-44 kDa
GenBank accession numberBC096192
Gene Symbol HOXB1
Gene ID (NCBI) 3211
RRIDAB_2878899
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP14653
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HOXB1, also named as HOX2I, belongs to the Antp homeobox family and the Labial subfamily. HOXB1 is sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. HOXB1 acts on the anterior body structures. Establishment of the spatial colinearity in the embryo is directly controlled by the Hox genes themselves. 21653-1-AP is a rabbit polyclonal antibody raised against residues near the N-terminus of human HOXB1.

Protocols

Product Specific Protocols
WB protocol for HOXB1 antibody 21653-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols