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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using HOXC10 Polyclonal antibody (12025-1-AP)

    WB analysis of mouse heart using 12025-1-AP (same clone as 12025-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 12025-1-AP (HOXC10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12025-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using HOXC10 Polyclonal antibody (12025-1-AP)

    WB analysis using 12025-1-AP (same clone as 12025-1-PBS)

    C2C12 cells were subjected to SDS PAGE followed by western blot with 12025-1-AP (HOXC10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12025-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using HOXC10 Polyclonal antibody (12025-1-AP)

    WB analysis of A549 using 12025-1-AP (same clone as 12025-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 12025-1-AP (HOXC10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12025-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HOXC10 Polyclonal antibody (12025-1-AP)

    IF Staining of HepG2 using 12025-1-AP (same clone as 12025-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12025-1-AP (HOXC10 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12025-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

HOXC10 Polyclonal antibody, PBS Only

HOXC10 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 12025-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12025-1-PBS targets HOXC10 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HOXC10 fusion protein Ag2655 相同性解析による交差性が予測される生物種
Full Name homeobox C10
Calculated molecular weight 342 aa, 38 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC001293
Gene Symbol HOXC10
Gene ID (NCBI) 3226
RRIDAB_2119611
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NYD6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

HOXC10, also named as Homeo box C10, is a 342 amino acid protein. The calculated molecular weight of HOXC10 is 38 kDa, but HOXC10 is detected about 50 kDa (PMID: 12853486)