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HOXC4 Polyclonal antibody

HOXC4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 14321-1-AP

Synonyms

HOX3E, HOX3, Homeobox protein Hox-C4, Homeobox protein Hox-3E, cp19

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HOXC4 Polyclonal antibody (14321-1-AP)

    WB analysis using 14321-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14321-1-AP (HOXC4 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using HOXC4 Polyclonal antibody (14321-1-AP)

    WB analysis of Jurkat using 14321-1-AP

    WB result of HOXC4 antibody (14321-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HOXC4 transfected Jurkat cells.

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Tested Applications

Positive WB detected inJurkat cells, PC-12 cells, U-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14321-1-AP targets HOXC4 in WB, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HOXC4 fusion protein Ag5563 相同性解析による交差性が予測される生物種
Full Name homeobox C4
Calculated molecular weight 30 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC050442
Gene symbol HOXC4
Gene ID (NCBI) 3221
RRIDAB_2233196
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The class I homeobox (HOX) family of homeodomain-containing transcription factors has 39 members in mammals. These genes are distributed throughout the genome in four clusters: HOXA (7p15), HOXB (17q21), HOXC (12q13), and HOXD (2q31). HOX genes are known to act as transcription factors, playing an essential regulatory role in the temporal and spatial development of the embryo. HOXC family members are not expressed in normal prostate tissue but increased expression of HOXC genes is commonly detected in prostate cancers. HOXC4 is expressed in GC B cells and HoxC4 expression is specifically up-regulated by the same stimuli that induce AID expression (LPS, CD154 and IL-4). The calculated molecular weight of HOXC4 is 30 kDa. Sometimes higher molecular weight around 39 kDa can also be observed, which may be a modified variant of HOXC4 (PMID: 23178148, 32012197, 35400027).

Protocols

Product Specific Protocols
WB protocol for HOXC4 antibody 14321-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncotarget

Long noncoding RNA uc.345 promotes tumorigenesis of pancreatic cancer by upregulation of hnRNPL expression.

Authors - Chao Liu
View Article