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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HOXD13 Polyclonal antibody

HOXD13 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18736-1-AP

Synonyms

BDE, BDSD, homeobox D13, Homeobox protein Hox 4I, Homeobox protein Hox D13, HOX4I, HOXD13, SPD

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using HOXD13 Polyclonal antibody (18736-1-AP)

    WB analysis of A549 using 18736-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 18736-1-AP (HOXD13 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using HOXD13 Polyclonal antibody (18736-1-AP)

    WB analysis of mouse lung using 18736-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 18736-1-AP (HOXD13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using HOXD13 Polyclonal antibody (18736-1-AP)

    WB analysis of mouse brain using 18736-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 18736-1-AP (HOXD13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using HOXD13 Polyclonal antibody (18736-1-AP)

    WB analysis of Jurkat using 18736-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 18736-1-AP (HOXD13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using HOXD13 Polyclonal antibody (18736-1-AP)

    WB analysis of rat brain using 18736-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 18736-1-AP (HOXD13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A549 cells using HOXD13 Polyclonal antibody (18736-1-AP)

    IP experiment of A549 using 18736-1-AP

    IP result of anti-HOXD13 (IP:18736-1-AP, 4ug; Detection:18736-1-AP 1:500) with A549 cells lysate 1600ug.

  • Immunohistochemistry (IHC) staining of human brain tissue using HOXD13 Polyclonal antibody (18736-1-AP)

    IHC staining of human brain using 18736-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 18736-1-AP (HOXD13 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using HOXD13 Polyclonal antibody (18736-1-AP)

    IHC staining of human brain using 18736-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 18736-1-AP (HOXD13 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using HOXD13 Polyclonal antibody (18736-1-AP)

    IHC staining of human hepatocirrhosis using 18736-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 18736-1-AP (HOXD13 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using HOXD13 Polyclonal antibody (18736-1-AP)

    IHC staining of human hepatocirrhosis using 18736-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 18736-1-AP (HOXD13 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HOXD13 Polyclonal antibody (18736-1-AP)

    IHC staining of human liver cancer using 18736-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 18736-1-AP (HOXD13 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HOXD13 Polyclonal antibody (18736-1-AP)

    IHC staining of human liver cancer using 18736-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 18736-1-AP (HOXD13 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inA549 cells, rat brain tissue, Jurkat cells, mouse lung tissue, mouse brain tissue
Positive IP detected inA549 cells
Positive IHC detected inhuman brain tissue, human hepatocirrhosis tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

18736-1-AP targets HOXD13 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name homeobox D13
Calculated molecular weight 343 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC148863
Gene symbol HOXD13
Gene ID (NCBI) 3239
RRIDAB_2119926
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HOXD13, also named as HOX4I, belongs to the Abd-B homeobox family. HOXD13 is a sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Mutation of HOXD13 will cause synpolydactyly (SPD), brachydactyly type D (BDD), syndactyly type 5brachydactyly-syndactyly syndrome (BDSD) or brachydactyly type E (BDE).Present polyclonal anti-HOX13D antibody(18736-1-AP) is produced by immunizing animals with part of N-terminus chain of HOX13D and detect a 36-kDa band in cell and tissues.

Protocols

Product Specific Protocols
WB protocol for HOXD13 antibody 18736-1-APDownload protocol
IHC protocol for HOXD13 antibody 18736-1-APDownload protocol
IP protocol for HOXD13 antibody 18736-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Mol Pharm

Sialic Acid-Functionalized PEG-PLGA Microspheres Loading Mitochondrial Targeting Modified Curcumin for Acute Lung Injury Therapy.

Authors - Fei-Yang Jin
View Article
mouseWB

Cell Death Dis

A novel Hoxd13 mutation causes synpolydactyly and promotes osteoclast differentiation by regulating pSmad5/p65/c-Fos/Rank axis

Authors - Lishan Zhang
View Article