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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HOXD13 Polyclonal antibody

HOXD13 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23520-1-AP

Synonyms

BDE, BDSD, homeobox D13, Homeobox protein Hox 4I, Homeobox protein Hox D13, HOX4I, HOXD13, SPD

Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using HOXD13 Polyclonal antibody (23520-1-AP)

    WB analysis of HL-60 using 23520-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 23520-1-AP (HOXD13 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using HOXD13 Polyclonal antibody (23520-1-AP)

    WB analysis of A549 using 23520-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 23520-1-AP (HOXD13 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using HOXD13 Polyclonal antibody (23520-1-AP)

    WB analysis of HL-60 using 23520-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 23520-1-AP (HOXD13 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using HOXD13 Polyclonal antibody (23520-1-AP)

    IF Staining of A549 using 23520-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 23520-1-AP (HOXD13 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHL-60 cells, A549 cells
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23520-1-AP is HOXD13 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HOXD13 fusion protein Ag20093 相同性解析による交差性が予測される生物種
Full Name homeobox D13
Calculated molecular weight 343 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC148863
Gene symbol HOXD13
Gene ID (NCBI) 3239
RRIDAB_2879291
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HOXD13 antibody 23520-1-APDownload protocol
IF protocol for HOXD13 antibody 23520-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols