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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HOXD9 Polyclonal antibody

HOXD9 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20560-1-AP

Synonyms

homeobox D9, Homeobox protein Hox 4C, Homeobox protein Hox 5.2, Homeobox protein Hox D9, Hox 4.3, Hox 5.2, HOX4, HOX4C, HOXD9

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using HOXD9 Polyclonal antibody (20560-1-AP)

    WB analysis of HeLa using 20560-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20560-1-AP (HOXD9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HOXD9 Polyclonal antibody (20560-1-AP)

    IF Staining of HeLa using 20560-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 20560-1-AP (HOXD9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

20560-1-AP targets HOXD9 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name homeobox D9
Calculated molecular weight 36 kDa
Observed molecular weight 32-37 kDa
GenBank accession numberNM_014213
Gene symbol HOXD9
Gene ID (NCBI) 3235
RRIDAB_2878702
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HOXD9 antibody 20560-1-APDownload protocol
IF protocol for HOXD9 antibody 20560-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

World J Surg Oncol

Homeobox D9 drives the malignant phenotypes and enhances the Programmed death ligand-1 expression in non-small cell lung cancer cells via binding to Angiopoietin-2 promoter

Authors - Jiabei He
View Article