Haptoglobin Polyclonal antibody

Name: Haptoglobin Polyclonal antibody
Brand: Proteintech
SKU: 16665-1-AP

Haptoglobin Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 16665-1-AP

Synonyms

HP, Zonulin, HPA1S, HP2 ALPHA 2, HAPT

Validation Data Gallery

Filter:

AllWBIPIHC

WB analysis of HEK-293 using 16665-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 16665-1-AP (Haptoglobin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 16665-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16665-1-AP (Haptoglobin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 16665-1-AP
Human plasma were subjected to SDS PAGE followed by western blot with 16665-1-AP (Haptoglobin antibody) at dilution of 1:18000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 16665-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 16665-1-AP (Haptoglobin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 16665-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 16665-1-AP (Haptoglobin antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 16665-1-AP
IP result of anti-Haptoglobin (IP:16665-1-AP, 4ug; Detection:16665-1-AP 1:500) with HepG2 cells lysate 2000ug.
IHC staining of human liver cancer using 16665-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16665-1-AP (Haptoglobin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 16665-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16665-1-AP (Haptoglobin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 16665-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16665-1-AP (Haptoglobin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 16665-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16665-1-AP (Haptoglobin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 16665-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16665-1-AP (Haptoglobin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 16665-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16665-1-AP (Haptoglobin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 16665-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse serum, HEK-293 cells, human liver tissue, human plasma, rat plasma
Positive IP detected in	HepG2 cells
Positive IHC detected in	human liver cancer tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 6 publications below
IHC	See 3 publications below
IF	See 1 publications below

Product Information

16665-1-AP targets Haptoglobin in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Haptoglobin fusion protein Ag10143 相同性解析による交差性が予測される生物種
Full Name	haptoglobin
Calculated molecular weight	281aa,31 kDa; 228aa,25 kDa
Observed molecular weight	42 kDa
GenBank accession number	BC058031
Gene symbol	HP
Gene ID (NCBI)	3240
RRID	AB_1939524
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HP(Haptoglobin) is also named as zonulin and belongs to the peptidase S1 family. HP, a plasma glycoprotein that binds free hemoglobin, has a tetrameric structure of 2 alpha(16 kDa and 9 kDa) and 2 beta(40 kDa) polypeptides that are covalently associated by disulfide bonds. In most species, apart from ruminants, Hp has a molecular mass of 100 kDa, consisting of two subunits of 40 kDa and two subunits of 9 kDa, although in a few species, such as man, genetic variant of Hp forms polymers of higher mass(PMID:2361363). Recent studies of haptoglobin show that certain oligosaccharide structures predominate in different diseases. For example, a highly-fucosylated structure is found in breast cancer and ovarian cancer, highly-sialylated structures in Crohn's disease and highly branched structures in alcoholic liver disease and fucosylated haptoglobin is a good serum marker for pancreatic cancer.(PMID:16385567).

Protocols

Product Specific Protocols
WB protocol for Haptoglobin antibody 16665-1-AP	Download protocol
IHC protocol for Haptoglobin antibody 16665-1-AP	Download protocol
IP protocol for Haptoglobin antibody 16665-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	WB	Circ Res Haptoglobin enhances cardiac transplant rejection. Authors - Hua Shen View Article
	WB	Theranostics Interleukin-6 Induced "Acute" Phenotypic Microenvironment Promotes Th1 Anti-Tumor Immunity in Cryo-Thermal Therapy Revealed By Shotgun and Parallel Reaction Monitoring Proteomics. Authors - Ting Xue View Article
human	IHC	Clin Transl Med Single-cell transcriptomes reveal heterogeneity of high-grade serous ovarian carcinoma. Authors - Qian Hao View Article
		Antioxid Redox Signal Control of Oxidative Stress and Inflammation in Sickle Cell Disease with the Nrf2 Activator Dimethyl Fumarate. Authors - John D Belcher View Article
human	WB	World J Gastroenterol N-linked glycoproteomic profiling in esophageal squamous cell carcinoma Authors - Qi-Wei Liu View Article
human	IHC	Oncol Rep Proteomic-based analysis for identification of potential serum biomarkers in gallbladder cancer. Authors - Tan Yi Y View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Haptoglobin Polyclonal antibody

Haptoglobin Polyclonal Antibody for WB, IHC, IP, ELISA

Validation Data Gallery

WB analysis of HEK-293 using 16665-1-AP

WB analysis using 16665-1-AP

WB analysis using 16665-1-AP

WB analysis of human liver using 16665-1-AP

WB analysis of human liver using 16665-1-AP

IP experiment of HepG2 using 16665-1-AP

IHC staining of human liver cancer using 16665-1-AP

IHC staining of human liver cancer using 16665-1-AP

IHC staining of human liver cancer using 16665-1-AP

IHC staining of human liver cancer using 16665-1-AP

IHC staining of human lung cancer using 16665-1-AP

IHC staining of human lung cancer using 16665-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Haptoglobin enhances cardiac transplant rejection.

Interleukin-6 Induced "Acute" Phenotypic Microenvironment Promotes Th1 Anti-Tumor Immunity in Cryo-Thermal Therapy Revealed By Shotgun and Parallel Reaction Monitoring Proteomics.

Single-cell transcriptomes reveal heterogeneity of high-grade serous ovarian carcinoma.

Control of Oxidative Stress and Inflammation in Sickle Cell Disease with the Nrf2 Activator Dimethyl Fumarate.

N-linked glycoproteomic profiling in esophageal squamous cell carcinoma

Proteomic-based analysis for identification of potential serum biomarkers in gallbladder cancer.