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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HPD Monoclonal antibody

HPD Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3C9G2

Cat no : 67552-1-Ig

Synonyms

4 HPPD, 4HPPD, GLOD3, HPD, HPPDase, PPD

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HPD Monoclonal antibody (67552-1-Ig)

    WB analysis using 67552-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67552-1-Ig (HPD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig liver tissue using HPD Monoclonal antibody (67552-1-Ig)

    WB analysis of pig liver using 67552-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67552-1-Ig (HPD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using HPD Monoclonal antibody (67552-1-Ig)

    WB analysis using 67552-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67552-1-Ig (HPD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HPD Monoclonal antibody (67552-1-Ig)

    IHC staining of human liver cancer using 67552-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67552-1-Ig (HPD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HPD Monoclonal antibody (67552-1-Ig)

    IHC staining of human liver cancer using 67552-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67552-1-Ig (HPD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, pig liver tissue, rat liver tissue, HepG2 cells, HEK-293 cells, HSC-T6 cells, mouse liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67552-1-Ig targets HPD in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Cited Reactivityhuman, zebrafish
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen HPD fusion protein Ag10928 相同性解析による交差性が予測される生物種
Full Name 4-hydroxyphenylpyruvate dioxygenase
Calculated molecular weight 393 aa, 45 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC024287
Gene symbol HPD
Gene ID (NCBI) 3242
RRIDAB_2882766
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HPD antibody 67552-1-IgDownload protocol
IHC protocol for HPD antibody 67552-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,zebrafishWB

Pest Manag Sci

In vivo fluorescent screening for HPPD-targeted herbicide discovery

Authors - Yi-Xuan Fu
View Article
humanIHC

Life Sci

Homogentisic acid metabolism inhibits papillary thyroid carcinoma proliferation through ROS and p21-induced cell cycle arrest

Authors - Ruiwang Xie
View Article