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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HPDL Polyclonal antibody (20777-1-AP)

    WB analysis using 20777-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20777-1-AP (HPDL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using HPDL Polyclonal antibody (20777-1-AP)

    WB analysis of L02 using 20777-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 20777-1-AP (HPDL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using HPDL Polyclonal antibody (20777-1-AP)

    WB analysis of L02 using 20777-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 20777-1-AP (HPDL antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using HPDL Polyclonal antibody (20777-1-AP)

    IHC staining of human stomach using 20777-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 20777-1-AP (HPDL antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using HPDL Polyclonal antibody (20777-1-AP)

    IHC staining of human stomach using 20777-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 20777-1-AP (HPDL antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using HPDL Polyclonal antibody (20777-1-AP)

    IHC staining of human colon using 20777-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 20777-1-AP (HPDL antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using HPDL Polyclonal antibody (20777-1-AP)

    IHC staining of human colon using 20777-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 20777-1-AP (HPDL antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using HPDL Polyclonal antibody (20777-1-AP)

    IF Staining of Caco-2 using 20777-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using HPDL antibody (20777-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

HPDL Polyclonal antibody

HPDL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20777-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

HPD-like protein, Glyoxalase domain-containing protein 1, GLOXD1, EC:1.13.-.-, 4-hydroxyphenylpyruvate dioxygenase-like protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, L02 cells
Positive IHC detected inhuman stomach tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCaco-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

20777-1-AP targets HPDL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HPDL fusion protein Ag14773 相同性解析による交差性が予測される生物種
Full Name 4-hydroxyphenylpyruvate dioxygenase-like
Calculated molecular weight 371 aa, 39 kDa
Observed molecular weight 35-39 kDa
GenBank accession numberBC007293
Gene Symbol HPDL
Gene ID (NCBI) 84842
RRIDAB_10697807
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96IR7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HPDL antibody 20777-1-APDownload protocol
IHC protocol for HPDL antibody 20777-1-APDownload protocol
IF protocol for HPDL antibody 20777-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Gene

Two novel heterozygous HPDL variants in a Chinese family with a neurodevelopmental disorder with progressive spasticity and brain white matter abnormalities

Authors - Yuanxuan Ma
View Article