HPDL Polyclonal antibody
HPDL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 20777-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, L02 cells |
Positive IHC detected in | human stomach tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | Caco-2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
20777-1-AP targets HPDL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | HPDL fusion protein Ag14773 相同性解析による交差性が予測される生物種 |
Full Name | 4-hydroxyphenylpyruvate dioxygenase-like |
Calculated molecular weight | 371 aa, 39 kDa |
Observed molecular weight | 35-39 kDa |
GenBank accession number | BC007293 |
Gene symbol | HPDL |
Gene ID (NCBI) | 84842 |
RRID | AB_10697807 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for HPDL antibody 20777-1-AP | Download protocol |
IHC protocol for HPDL antibody 20777-1-AP | Download protocol |
IF protocol for HPDL antibody 20777-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |