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Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using HPGD Polyclonal antibody (11035-1-AP)

    WB analysis of human placenta using 11035-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 11035-1-AP (HPGD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of human placenta tissue using HPGD Polyclonal antibody (11035-1-AP)

    IP experiment of human placenta using 11035-1-AP

    IP result of anti-HPGD (IP:11035-1-AP, 4ug; Detection:11035-1-AP 1:300) with human placenta tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HPGD Polyclonal antibody (11035-1-AP)

    IHC staining of human colon cancer using 11035-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11035-1-AP (HPGD antibody) at dilution of 1:50 (under 25x lens).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using HPGD Polyclonal antibody (11035-1-AP)

    IF Staining of A549 using 11035-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 11035-1-AP (HPGD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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HPGD Polyclonal antibody

HPGD Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11035-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Eicosanoid/docosanoid dehydrogenase [NAD(+)], EC:1.1.1.141, EC:1.1.1.-, EC:1.1.1.232, 15-PGDH, 15-hydroxyprostaglandin dehydrogenase [NAD(+)]

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman placenta tissue
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11035-1-AP targets HPGD in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HPGD fusion protein Ag1499 相同性解析による交差性が予測される生物種
Full Name hydroxyprostaglandin dehydrogenase 15-(NAD)
Calculated molecular weight 29 kDa
Observed molecular weight 25-28 kDa
GenBank accession numberBC018986
Gene Symbol HPGD
Gene ID (NCBI) 3248
RRIDAB_2120407
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP15428
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HPGD, also named as PGDH (15-hydroxyprostaglandin dehydrogenase), a prostaglandin-degrading enzyme that physiologically antagonizes COX-2 (PMID:15574495). HPGD inhibits in vivo proferation of colon cancer cells (PMID:24760190). HPGD has five isoforms with the molecular mass of 29, 21, 19, 16 and 15 kDa.

Protocols

Product Specific Protocols
WB protocol for HPGD antibody 11035-1-APDownload protocol
IHC protocol for HPGD antibody 11035-1-APDownload protocol
IF protocol for HPGD antibody 11035-1-APDownload protocol
IP protocol for HPGD antibody 11035-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF

Int J Mol Sci

The Enzyme 15-Hydroxyprostaglandin Dehydrogenase Inhibits a Shift to the Mesenchymal Pattern of Trophoblasts and Decidual Stromal Cells Accompanied by Prostaglandin Transporter in Preeclampsia

Authors - Huiyuan Pang
View Article