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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HPGD Monoclonal antibody (66798-1-Ig)

    WB analysis using 66798-1-Ig (same clone as 66798-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66798-1-Ig (HPGD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66798-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human small intestine tissue using HPGD Monoclonal antibody (66798-1-Ig)

    WB analysis of human small intestine using 66798-1-Ig (same clone as 66798-1-PBS)

    human small intestine tissue were subjected to SDS PAGE followed by western blot with 66798-1-Ig (HPGD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66798-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using HPGD Monoclonal antibody (66798-1-Ig)

    IHC staining of human colon using 66798-1-Ig (same clone as 66798-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66798-1-Ig (HPGD antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66798-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using HPGD Monoclonal antibody (66798-1-Ig)

    IHC staining of human colon using 66798-1-Ig (same clone as 66798-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66798-1-Ig (HPGD antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66798-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HPGD Monoclonal antibody (66798-1-Ig)

    IHC staining of human colon cancer using 66798-1-Ig (same clone as 66798-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66798-1-Ig (HPGD antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66798-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HPGD Monoclonal antibody (66798-1-Ig)

    IHC staining of human colon cancer using 66798-1-Ig (same clone as 66798-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66798-1-Ig (HPGD antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66798-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HPGD Monoclonal antibody (66798-1-Ig)

    IHC staining of human lung cancer using 66798-1-Ig (same clone as 66798-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66798-1-Ig (HPGD antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66798-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HPGD Monoclonal antibody (66798-1-Ig)

    IHC staining of human lung cancer using 66798-1-Ig (same clone as 66798-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66798-1-Ig (HPGD antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66798-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using HPGD Monoclonal antibody (66798-1-Ig)

    IF Staining of A549 using 66798-1-Ig (same clone as 66798-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using 66798-1-Ig (HPGD antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66798-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using HPGD Monoclonal antibody (66798-1-Ig)

    FC experiment of A549 using 66798-1-Ig (same clone as 66798-1-PBS)

    1X10^6 A549 cells were intracellularly stained with 0.2 ug Anti-Human HPGD (66798-1-Ig, Clone:3F1F5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66798-1-PBS in a different storage buffer formulation.

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HPGD Monoclonal antibody, PBS Only

HPGD Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 66798-1-PBS
Clone No.3F1F5

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

15 PGDH, HPGD, PGDH, PGDH1, Prostaglandin dehydrogenase 1, SDR36C1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66798-1-PBS targets HPGD in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HPGD fusion protein Ag1499 相同性解析による交差性が予測される生物種
Full Name hydroxyprostaglandin dehydrogenase 15-(NAD)
Calculated molecular weight 29 kDa
Observed molecular weight 25-28 kDa
GenBank accession numberBC018986
Gene Symbol HPGD
Gene ID (NCBI) 3248
RRIDAB_2861358
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP15428
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

HPGD, also named as PGDH (15-hydroxyprostaglandin dehydrogenase), a prostaglandin-degrading enzyme that physiologically antagonizes COX-2 (PMID:15574495). HPGD inhibits in vivo proferation of colon cancer cells (PMID:24760190). HPGD has five isoforms with the molecular mass of 29, 21, 19, 16 and 15 kDa.