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  • Featured Product
  • KD/KO Validated

HPRT1 Monoclonal antibody, PBS Only

HPRT1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C3D1

Cat no : 67518-1-PBS

Synonyms

HGPRT, HGPRTase, HPRT, HPRT1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HPRT1 Monoclonal antibody (67518-1-Ig)

    WB analysis using 67518-1-Ig (same clone as 67518-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67518-1-Ig (HPRT1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. HPRT1 deficient cell line Sp2/0 was served as a negative control. This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using HPRT1 Monoclonal antibody (67518-1-Ig)

    WB analysis of HEK-293 using 67518-1-Ig (same clone as 67518-1-PBS)

    WB result of HPRT1 antibody (67518-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HPRT1 transfected HEK-293 cells. This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using HPRT1 Monoclonal antibody (67518-1-Ig)

    IHC staining of mouse liver using 67518-1-Ig (same clone as 67518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67518-1-Ig (HPRT1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using HPRT1 Monoclonal antibody (67518-1-Ig)

    IHC staining of mouse liver using 67518-1-Ig (same clone as 67518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67518-1-Ig (HPRT1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using HPRT1 Monoclonal antibody (67518-1-Ig)

    IHC staining of human endometrial cancer using 67518-1-Ig (same clone as 67518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 67518-1-Ig (HPRT1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using HPRT1 Monoclonal antibody (67518-1-Ig)

    IHC staining of human endometrial cancer using 67518-1-Ig (same clone as 67518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 67518-1-Ig (HPRT1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HPRT1 Monoclonal antibody (67518-1-Ig)

    IHC staining of human liver cancer using 67518-1-Ig (same clone as 67518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67518-1-Ig (HPRT1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HPRT1 Monoclonal antibody (67518-1-Ig)

    IHC staining of human colon cancer using 67518-1-Ig (same clone as 67518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67518-1-Ig (HPRT1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HPRT1 Monoclonal antibody (67518-1-Ig)

    IHC staining of human colon cancer using 67518-1-Ig (same clone as 67518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67518-1-Ig (HPRT1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HPRT1 Monoclonal antibody (67518-1-Ig)

    IF Staining of HeLa using 67518-1-Ig (same clone as 67518-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 67518-1-Ig (HPRT1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67518-1-PBS targets HPRT1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HPRT1 fusion protein Ag7044 相同性解析による交差性が予測される生物種
Full Name hypoxanthine phosphoribosyltransferase 1
Calculated molecular weight 25 kDa
Observed molecular weight 24-28 kDa
GenBank accession numberBC000578
Gene symbol HPRT1
Gene ID (NCBI) 3251
RRIDAB_2882738
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HPRT1, also named as HGPRT, plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutation of HPRT1 is associated with Lesch-Nyhan syndrome (LNS) which is an X-linked inherited neurogenetic disorder of purine metabolism. It has been reported that HPRT1 also plays an important role in HPRT-related gout. 67518-1-Ig antibody detects the protein around 28 kDa in SDS-PAGE. And HPRT1 deficient cell line Sp2/0 was served as a negative control in PTG's results.(PMID:20544509, 28045594, 27379977)