HPS5 Polyclonal antibody
HPS5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 13901-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse liver tissue, mouse spleen tissue |
Positive IHC detected in | human placenta tissue, human heart tissue, human lung tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2400 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 6 publications below |
Product Information
13901-1-AP targets HPS5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | HPS5 fusion protein Ag4867 相同性解析による交差性が予測される生物種 |
Full Name | Hermansky-Pudlak syndrome 5 |
Calculated molecular weight | 1129 aa, 127 kDa |
Observed molecular weight | 135-141 kDa |
GenBank accession number | BC033640 |
Gene symbol | HPS5 |
Gene ID (NCBI) | 11234 |
RRID | AB_2120685 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Hermansky-Pudlak syndrome 5 protein (HPS-5), also named Ruby-eye protein 2 homolog (Ru2), plays a role in organelle biogenesis associated with melanosomes, platelet dense granules, and lysosomes. This protein interacts with Hermansky-Pudlak syndrome 6 protein and may interact with the cytoplasmic domain of integrin, alpha-3. Mutations in this gene are associated with Hermansky-Pudlak syndrome type 5.
Protocols
Product Specific Protocols | |
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WB protocol for HPS5 antibody 13901-1-AP | Download protocol |
IHC protocol for HPS5 antibody 13901-1-AP | Download protocol |
IF protocol for HPS5 antibody 13901-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Blood vWF maturation and release are controlled by two regulators of Weibel-Palade body biogenesis: exocyst and BLOC-2. | ||
Am J Hum Genet A BLOC-1 mutation screen reveals that PLDN is mutated in Hermansky-Pudlak Syndrome type 9. | ||
J Invest Dermatol Clinical, molecular, and cellular features of non-Puerto Rican Hermansky-Pudlak syndrome patients of Hispanic descent. | ||
Pigment Cell Melanoma Res A BLOC-1 mutation screen reveals a novel BLOC1S3 mutation in Hermansky-Pudlak Syndrome type 8. | ||
PLoS One Cellular and molecular defects in a patient with Hermansky-Pudlak syndrome type 5. | ||
Front Genet Oculocutaneous albinism and bleeding diathesis due to a novel deletion in the HPS3 gene |