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  • Featured Product
  • KD/KO Validated

HPSE Monoclonal antibody, PBS Only

HPSE Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D8B8

Cat no : 66226-1-PBS

Synonyms

Endo glucoronidase, HEP, heparanase, Heparanase 1, HPA, Hpa1, HPR1, HPSE, HPSE1, HSE1

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using HPSE Monoclonal antibody (66226-1-Ig)

    WB analysis of HepG2 using 66226-1-Ig (same clone as 66226-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66226-1-Ig (HPSE Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66226-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HPSE Monoclonal antibody (66226-1-Ig)

    IHC staining of human liver cancer using 66226-1-Ig (same clone as 66226-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66226-1-Ig (HPSE Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66226-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HPSE Monoclonal antibody (66226-1-Ig)

    IHC staining of human liver cancer using 66226-1-Ig (same clone as 66226-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66226-1-Ig (HPSE Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66226-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HPSE Monoclonal antibody (66226-1-Ig)

    IF Staining of HepG2 using 66226-1-Ig (same clone as 66226-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66226-1-Ig(HPSE antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66226-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66226-1-PBS targets HPSE in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HPSE fusion protein Ag10067 相同性解析による交差性が予測される生物種
Full Name heparanase
Calculated molecular weight 543 aa, 61 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC051321
Gene symbol HPSE
Gene ID (NCBI) 10855
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

HPSE(Heparanase) is also named as HEP, HPA, HPA1, HPR1, HPSE1, HSE1 and belongs to the glycosyl hydrolase 79 family. It is a endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. HPSE is essential in the disassembly of the extracellular matrix (ECM) by invading cells. It has 3 isoforms produced by alternative splicing with the molecular weight of 61 kDa, 55 kDa and 53 kDa. The full length protein has six glycosylation sites. The cleavage of the 65 kDa form leads to the generation of a linker peptide, and 8 kDa and 50 kDa products. The active form, the 8/50 kDa heterodimer, is resistant to degradation and glycosylation of the 50 kDa subunit appears to be essential for its solubility.