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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using HPSE Recombinant antibody (84562-1-RR)

    WB analysis of HeLa using 84562-1-RR (same clone as 84562-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 84562-1-RR (HPSE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84562-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using HPSE Recombinant antibody (84562-1-RR)

    IHC staining of human placenta using 84562-1-RR (same clone as 84562-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84562-1-RR (HPSE antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84562-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using HPSE Recombinant antibody (84562-1-RR)

    IHC staining of human placenta using 84562-1-RR (same clone as 84562-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84562-1-RR (HPSE antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84562-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using HPSE Recombinant antibody (84562-1-RR)

    FC experiment of HepG2 using 84562-1-RR (same clone as 84562-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug HPSE Recombinant Antibody (84562-1-RR, Clone:241651E3) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84562-1-PBS in a different storage buffer formulation.

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HPSE Recombinant antibody, PBS Only

HPSE Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 84562-1-PBS
Clone No.241651E3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

Heparanase, HEP, Endo-glucoronidase, Endo glucoronidase, EC:3.2.1.166

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84562-1-PBS targets HPSE in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HPSE fusion protein Ag21479 相同性解析による交差性が予測される生物種
Full Name heparanase
Calculated molecular weight 543 aa, 61 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC051321
Gene Symbol HPSE
Gene ID (NCBI) 10855
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9Y251
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HPSE(Heparanase) is also named as HEP, HPA, HPA1, HPR1, HPSE1, HSE1 and belongs to the glycosyl hydrolase 79 family. It is a endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. HPSE is essential in the disassembly of the extracellular matrix (ECM) by invading cells. It has 3 isoforms produced by alternative splicing with the molecular weight of 61 kDa, 55 kDa and 53 kDa. The full length protein has six glycosylation sites. The cleavage of the 65 kDa form leads to the generation of a linker peptide, and 8 kDa and 50 kDa products. The active form, the 8/50 kDa heterodimer, is resistant to degradation and glycosylation of the 50 kDa subunit appears to be essential for its solubility.